Rosacea Infection On Our Skin Case Study

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Rosacea Infection on Our Skin
The human microbiome is the cumulative genomes of all the different types of microbes ranging from bacteria, fungi, and viruses that live in and outside of the human body. We have many mutualistic, commensal, and even detrimental microbes living in our bodies. There are commensal microbes that sometimes are also responsible for the infection due to the microbe containing a pathogenic strain. In our laboratory, there appears to be 40 patients that are experiencing a visible skin infection on their face, and the infection does not seem to respond to the current antimicrobial treatment. Upon observation of this skin infection, it appears to be a rosacea infection. To confirm and verify the identity of the infection,
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The culturing of the microorganism is not always necessary with the molecular approach. It produces rapid results and is usually more accurate than the classical method. In the molecular approach, we would use PCR to amplify the quantity of nucleic acids present in the specimen collection. It was discovered that rosacea had high levels that involved T-cells. This signifies that the expression of T-cell activation elevated and infiltrates rosacea (Buhl, T., 2015). Pulse-field gel electrophoresis will then help to separate the DNA fragments by size to help differentiate the subtypes of rosacea. Ribotyping is another genotypic method that can be used and is a great indicator to compare the genetic makeup of the microbe. It is great for diagnosing an infection. The rRNA is isolated, sequenced, and analyzed from the sample. Studies have demonstrated the major roles that antimicrobial peptides and the toll-like receptors 2 pathway in rosacea (Chang, A., 2015). Through isolation and sequencing of rosacea, the genetic component of rosacea is consistent with the inflammatory nature of the disease. The toll-like receptors 2 is responsible and triggers the inflammation caused in rosacea. Mass spectrometry is used to determine the structure and analyze the composition of chemical compounds and biological molecules in the microbe. To further identify the pathogen, we can perform an immunologic test for the presence of specific antibodies to a suspected pathogen to analyze for an antibody response. The characteristics of antibodies seen macroscopically and microscopically can help detect, identify, and quantify antibodies or antigens if the patients had encountered the microbes before. This is a very specific method that can assist in determining the identity of the pathogen. By using the molecular method, it is much more specific to reveal genetic and

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