The participants received nutritional orientation …show more content…
Resveratrol (trans-resveratrol, 98% purified) and placebo were obtained from a manipulation pharmacy in Porto Alegre, RS, Brazil. Subjects were instructed to take the first supplement after the baseline measurements (day 1) and the last supplement at the end of 4 weeks (day 30). They were requested to maintain their normal lifestyle, physical activity and their usual diet throughout the study. The subjects were also instructed to abstain from foods containing substantial amounts of resveratrol (e.g. wine, red grapes, peanuts and berries) and were advised not to take any other food supplements during the …show more content…
The samples were loaded into a 96-well plate and diluted 1:2 with a co-substrate mixture containing NADPH, glutathione and glutathione reductase. The assay included negative and positive controls. Cumene hydroperoxide was then added to initiate the reaction and the consumption of NADPH was monitored at 340nm at five different moments. One unit of GPx is defined as 1ųmol of NADPH consumed per minute and the activity is represented as GPx units / mg protein. The assessment of CRP was measured in HCPA laboratory in accordance with the manufacturer’s instructions. The results are expressed in mg /