Many organisms have both beneficial and harmful bacteria. These bacteria have special defense mechanisms, virulence, against foreign nucleic acids by using plasmid DNA (Garneau, 2010). pAMP is a special plasmid that bacteria have for ampicillin resistance. With bacteria being able to spread this resistance, other possible harmful bacteria are able to use this to be able to resist ampicillin (Abramovitch, 2006). This creates a harmful or lethal environment for the organism that has the bacteria with the resistance gene (Abramovitch, 2006). In order to find specific sizes of the fragments, scientists use restriction enzymes to put the puzzle together (van der Lelie, 1999). Therefore, the purpose of this experiment is to construct a plasmid map using restriction enzymes.
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The pAMP sample runs through an agarose gel with the process of electrophoresis. If different restriction enzymes are used to cleave the pAMP, then during electrophoresis, the fragments will migrate down the agarose gel according to the fragment size. Using the results from the electrophoresis and a standard curve, the fragments can be measured according to the migration distance and put together a plasmid map. That being said, scientists are able to use restriction enzymes to figure out how the bacteria obtained the antibiotic virulence and come up with new solutions to halt the spread of enhanced
The pAMP sample runs through an agarose gel with the process of electrophoresis. If different restriction enzymes are used to cleave the pAMP, then during electrophoresis, the fragments will migrate down the agarose gel according to the fragment size. Using the results from the electrophoresis and a standard curve, the fragments can be measured according to the migration distance and put together a plasmid map. That being said, scientists are able to use restriction enzymes to figure out how the bacteria obtained the antibiotic virulence and come up with new solutions to halt the spread of enhanced