Essay on Relationship Between Temperature And Retention Time

1141 Words Mar 10th, 2016 5 Pages
Previously, for the first part of the experiment, it was hypothesized that the retention times of the eluates were invariably associated with the polarity of the compound; the more non-polar the compound, the faster it would be eluted out of the gas chromatogram. It was also hypothesized that the retention times decreased as the temperature in the gas column increased. In the first part of the experiment the relationship between the temperature and retention time, and the non-polarity of the molecule and the retention time was determined. As the temperature in the gas column increased the organic compounds eluted out of the gas chromatogram with a shorter retention time.
When the temperatures are higher than the organic compound’s boiling point, it would condense to liquid at a higher rate and eventually be eluted out of the gas chromatogram (2). The increased temperature allows the organic molecule to acquire kinetic energy to condense into a gas phase, also the interaction between the mobile and stationary phase could not withstand the kinetic energy and the eluates are eluted quicker (2). In vernier GC Mini, the stationary phase the coats the column is made up of non-polar molecule. Non-polar molecules are more soluble with compounds of its kind, likewise with polar molecules. In this lab, the column interacted with non-polar molecules more strongly and it caused the non-polar elute to be eluted slower (2). Also from the gas chromatographs, it was noticed that…

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