Myskowiak and Doums’ (2003) finding that refrigeration of P. terraenovae can affect the development and biometry of larval specimens after only one day of refrigeration suggests that a common forensic practice could be introducing significant error in to PMI estimations. Furthermore, the different relationships between duration of refrigeration, the stage refrigerated, and the time to development indicate that only L3 larvae return to their standard development rate after quiescence, indicating that a period of refrigeration has a lasting impact on the development of the specimen. The developmental sensitivity and increased mortality of pupating larvae to refrigeration is explained by the pupaes’ increased metabolic demands due to extensive tissue …show more content…
The basic foundation of the ADH concept is that there is a fixed quantity of metabolic activity, which is correlated with time and temperature, necessary to complete development, which is not reflective of the true developmental patterns found experimentally.
While Myskowiak and Doums (2002) briefly address the difference between diapause and quiescence, they never return to the concept or really explain its significance in context with the relationship of refrigeration and development. Ames and Turner (2003) revisit this point in their concluding remarks, suggesting that diapause, since it requires specific triggers for its activation and deactivation, is unlikely to be the physiological mechanism at work here, and instead it is likely that the flies are experiencing quiescence, indicating that it can not be assumed that no growth has occurred during cold