Recombinant Dna Research Paper

A DNA molecule made in vitro with segments from different sources is known as, Recombinant DNA. Recombinant DNA, otherwise known as rDNA, is an artificial DNA strand, created by combining gene sequences with one another. In order to create a rDNA you must first isolate the DNA donor, as well as the vector. Bacterial plasmids are commonly used, so for this explanation it will be used as the vector. The next step is to cut the DNA. This is possible with the help of restriction enzymes, which act as scissors cutting only at certain DNA targets sequences (restriction sites). Once the restriction enzymes cut the DNA into short nucleotide sequences it will then, in turn, create a set of restriction fragments. Now with these new double-stranded restriction fragments, at least one will be a sticky end (single-stranded end). The sticky ends on the fragments from one DNA source can base pair with complementary sticky ends on fragments …show more content…
In 1971 a gene-splicing experiment done by Paul Berg resulted in the first man-made recombinant DNA. Berg, however, did not continue with the next step of introducing recombinant DNA into another organism. The next step in continuing this process would be the insertion of the recombinant DNA into a bacteria, which was done in 1972 by Herbert Boyer, in collaboration with Stanley Cohen. The limitations of recombination size of genes and the ability to transfer the gene. In order for the recombination process to happen, the correct vector must be in use. A vector must have three sequences an origin of replication site, antibiotic resistance gene, and a multiple cloning site. Another thing is making sure the size of the DNA is correct. Specific traits might have more than one gene working together, so the size of DNA would actually be bigger, however bacteria and viruses can only carry small