Rat Model Of Oxidative Stress

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4.1 Introduction:

Stress is one of the most prevalent and life-threatening forms of mental illness affecting more than one fifth of the world’s population. It is always related to alterations of signaling pathways mainly the hypothalamic-pituitary-adrenal axis (Mikhai et al, 2014). Rat models are an obligatory tool to study the etiology and progression of behavioural and biochemical changes induced by oxidative stress. Restraint stress produces an imbalance between oxidants and antioxidants, which are the major cause for the pathogenesis of various neurodegenerative diseases. Oxidative neurodegeneration is a key factor in worsening the morphological responses and deficits in behavioural recoveries. Stress is known to elevate circulating
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The OFT apparatus was made of a large square shaped arena of 80 cm×80 cm by 40 cm high walls. The floor was marked into 25 equal square segments to allow quantification of locomotor activity. The segments were divided into outer, inner and a central region. A 40-W frosted bulb was suspended above the arena. The motor activity, exploring and emotional behaviour of the animal was measured in 5 minute test time. The acclimated rats were brought to the behavioural procedure room singly with experimental lights with doors closed. Proper Care was taken not to disturb, with sound or light. Each trial lasted for about 5min. After completion of the test, the rats were returned to the home cage. At the end of each trial, the arena was cleaned with 70% alcohol solution and dried with paper towel. Each rat was placed at the center of the arena and was observed for the following. Time spent in the center and the periphery of the arena (ambulation), standing on the hind legs with or without a support of the wall and the number of fecal pellets passed from the animal. The performance data of each rat were recorded and analyzed …show more content…
The effect of EE-ACL, A-Asarone and quercetin on restraint stress rats on open field test in the centre and periphery is shown in Figure. 4.1. Time spent in the centre of field is 25% decreased in stress group than control group. 30% of mean time is increased in EE-ACL group than stress group. A-Asarone group shows 20% decrease from control group and equal with stress group. Quercetin group spent time in centre more than 20% from stress group and equal to control group. The differences in the mean values among the treatment groups are statistically significant (p<

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