RESULTS AND DISCUSSIONS
Prior to capture the P.putida behavior in metabolic modelling experiments, Growth and productivity level of PHAs in Pseudomonas putida was assessed by various types of carbon sources in shake flask and batch cultivation. In the PHA production process, productivity level is influenced by the availability and types of carbon source
Production of PHA in shake flask condition
Shake flask cultivation of P.putida was carried out with various types of carbon sources. The carbon sources were categorized based on the nature of the substrates such as structurally related substrates Linoleic acid (Polyunsaturated fatty acid) Oleic acid(Mono saturated fatty acid), Lauric acid (Saturated fatty acids) Octanoic acid(Saturated …show more content…
It reached the maximum biomass of 2.32 g/L at 36 h and then the growth was retarded. Obtained final biomass was 1.32 fold higher than linoleic acid that produced maximum amount of PHA. Finally it yields 0.32 g/L of PHA with 14.8% PHA content from the resulting biomass which is lower than other carbon sources employed in shake flask cultivation. Polymer strongest band carbonyl ester also seen in 1742cm-1.It further confirmed mcl PHA also from the structurally unrelated carbon …show more content…
putida shows that an unusually long lag phase was observed (12 hours) when grows on glycerol (Glycerol mcl PHA REVIEW) .Escapa .et.al pinpointed that supplementing small amount of fatty acids or glucose, the length of lag phase can be minimized. Here, lag phase was eliminated by higher concentration of cells with nitrogen enriched complex medium as a inoculum. It can be clearly seen in fig maximum specific growth rate 0.6 h-1 and it is declined to 0.01h-1at 24 h . At 27 h maximum of 2.8 g/L biomass was achieved later, it was decreased to 2.8 g/L at 30h. Finally, 0.52 g/L of PHA was obtained from the 2.8 g/L of biomass with 15.3% PHA yield. When compare with glucose carbon allocated exclusively for biomass (PHA free biomass) was increased by 37 % further, PHA yield from total biomass was increased by 39 %. Higher productivity of PHA from glycerol was due to the reduced nature than glucose.Both biomass and PHA productivity level was higher than glucose.FTIR Showed the maximum relative absorbance at 1742 to confirm the polymer
Prior to capture the P.putida behavior in metabolic modelling experiments, Growth and productivity level of PHAs in Pseudomonas putida was assessed by various types of carbon sources in shake flask and batch cultivation. In the PHA production process, productivity level is influenced by the availability and types of carbon source
Production of PHA in shake flask condition
Shake flask cultivation of P.putida was carried out with various types of carbon sources. The carbon sources were categorized based on the nature of the substrates such as structurally related substrates Linoleic acid (Polyunsaturated fatty acid) Oleic acid(Mono saturated fatty acid), Lauric acid (Saturated fatty acids) Octanoic acid(Saturated …show more content…
It reached the maximum biomass of 2.32 g/L at 36 h and then the growth was retarded. Obtained final biomass was 1.32 fold higher than linoleic acid that produced maximum amount of PHA. Finally it yields 0.32 g/L of PHA with 14.8% PHA content from the resulting biomass which is lower than other carbon sources employed in shake flask cultivation. Polymer strongest band carbonyl ester also seen in 1742cm-1.It further confirmed mcl PHA also from the structurally unrelated carbon …show more content…
putida shows that an unusually long lag phase was observed (12 hours) when grows on glycerol (Glycerol mcl PHA REVIEW) .Escapa .et.al pinpointed that supplementing small amount of fatty acids or glucose, the length of lag phase can be minimized. Here, lag phase was eliminated by higher concentration of cells with nitrogen enriched complex medium as a inoculum. It can be clearly seen in fig maximum specific growth rate 0.6 h-1 and it is declined to 0.01h-1at 24 h . At 27 h maximum of 2.8 g/L biomass was achieved later, it was decreased to 2.8 g/L at 30h. Finally, 0.52 g/L of PHA was obtained from the 2.8 g/L of biomass with 15.3% PHA yield. When compare with glucose carbon allocated exclusively for biomass (PHA free biomass) was increased by 37 % further, PHA yield from total biomass was increased by 39 %. Higher productivity of PHA from glycerol was due to the reduced nature than glucose.Both biomass and PHA productivity level was higher than glucose.FTIR Showed the maximum relative absorbance at 1742 to confirm the polymer