In this model, neuropathic pain is induced by surgically implanting a polyethylene cuff (2 mm in length, inner diameter 0.7 mm) around the common branch of the sciatic nerve of rats.Sciatic nerve cuffs are made from thick-walled polyethylenetubing. Inner diameters of 0.026" and 0.028" arecreated by enlarging commercially available 0.023" tubing with reamingbits. Tubing are cut either into short (< 0.5 mm) segments orlonger segments of 2-5 mm in length(Mosconi and Kruger, 1996).
Procedure
• Sciatic nerves are exposed in the thigh using a Zeiss surgical microscope and freed from the connective tissue by sharp dissection with minimal involvement of the epineurial vasculature.
• Cuffs are applied to the nerve and after a brief blanching, the epineurial vessels filled and flow remained intact through closure of the incision.
• …show more content…
• Wounds are closed in two layers and the animals are permitted to recover before being returned to cages in the vivarium (Mosconi and Kruger, 1996).
Advantage: The neuropathic pain model is characterized by heat-hyperalgesia lasting for 3 weeks and mechanical allodynia lasting for 2 months.
Disadvantage: This model is difficult to
Procedure
• Sciatic nerves are exposed in the thigh using a Zeiss surgical microscope and freed from the connective tissue by sharp dissection with minimal involvement of the epineurial vasculature.
• Cuffs are applied to the nerve and after a brief blanching, the epineurial vessels filled and flow remained intact through closure of the incision.
• …show more content…
• Wounds are closed in two layers and the animals are permitted to recover before being returned to cages in the vivarium (Mosconi and Kruger, 1996).
Advantage: The neuropathic pain model is characterized by heat-hyperalgesia lasting for 3 weeks and mechanical allodynia lasting for 2 months.
Disadvantage: This model is difficult to