Methods:
To begin, our group acquired three premade vials of .1ml mammal blood with coagulant. We then labeled the vials numbers one, two, and three. In vial number one we added 1 ml of Stock 300 mM NaCl, and no distilled water. This resulted in a NaCl concentration of 1.8 percent. In the second vial we added .5 ml of Stock 300 mM of NaCl, and .5 ml of distilled water. This resulted in a NaCl concentration of .9 percent. In the third vial we did not add any Stock 300 mM of NaCl, and 1 ml of distilled water. Once the vials were at the proper NaCl concentration, we gently swirled the vials to completely mix the contents. We allowed it to sit for a minute, and observed. To begin with this section of the experiment, we acquired 3 leaves from the growing tip of an elodea plant. We then mounted each of the leaves on its own slide and placed a cover glass over the leaf. On the first slide we added distilled water and drew the water across the slide …show more content…
We determined that Vial number one was the cloudiest, and was a darker red, this was our hypotonic solution. Vial number two was decidedly less cloudy, and a slightly less deep red, this was our isotonic solution. Vial number three was very clear and the color did not change, we were able to read writing on our notebook page through the sample, this was our hypertonic solution. In the elodea plant portion of the experiment, we noticed that the third slide the cytoplasm was fitted to the cell wall, and had no more room to expand, this was our hypotonic solution. The second slide showed the cytoplasm sitting comfortably within the cell wall, with room to expand if it needed to, this was our isotonic solution. The first slide showed the cytoplasm considerable shrunken away from the cell wall and shrivelled, this was our hypertonic
To begin, our group acquired three premade vials of .1ml mammal blood with coagulant. We then labeled the vials numbers one, two, and three. In vial number one we added 1 ml of Stock 300 mM NaCl, and no distilled water. This resulted in a NaCl concentration of 1.8 percent. In the second vial we added .5 ml of Stock 300 mM of NaCl, and .5 ml of distilled water. This resulted in a NaCl concentration of .9 percent. In the third vial we did not add any Stock 300 mM of NaCl, and 1 ml of distilled water. Once the vials were at the proper NaCl concentration, we gently swirled the vials to completely mix the contents. We allowed it to sit for a minute, and observed. To begin with this section of the experiment, we acquired 3 leaves from the growing tip of an elodea plant. We then mounted each of the leaves on its own slide and placed a cover glass over the leaf. On the first slide we added distilled water and drew the water across the slide …show more content…
We determined that Vial number one was the cloudiest, and was a darker red, this was our hypotonic solution. Vial number two was decidedly less cloudy, and a slightly less deep red, this was our isotonic solution. Vial number three was very clear and the color did not change, we were able to read writing on our notebook page through the sample, this was our hypertonic solution. In the elodea plant portion of the experiment, we noticed that the third slide the cytoplasm was fitted to the cell wall, and had no more room to expand, this was our hypotonic solution. The second slide showed the cytoplasm sitting comfortably within the cell wall, with room to expand if it needed to, this was our isotonic solution. The first slide showed the cytoplasm considerable shrunken away from the cell wall and shrivelled, this was our hypertonic