Photosynthesis is a biochemical process from light using energy from sunlight and carbon dioxide from the atmosphere as reactants to produce oxygen and organic compounds like carbohydrates (Campbell et al. 2015). Photosynthesis takes place in photoautotrophs including all plants, algae, some kinds of bacteria, and protists. The simplified equation is: CO2 + H2O → carbohydrates + O2. (Sadava et al. 2014). It is broken down into two parts: light reaction and light-independent reaction (Campbell et al. 2015). In the light reaction, the pigments including chlorophylls a, chlorophylls b and carotenoids capture the light energy which is mainly visible light (400-700 nm) in the multiprotein complexes called photosystem within thylakoid membrane (Campbell
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2015). When DCPIP is oxidize d, it is blue; when DCPIP is reduced which means it snatches electrons from plastoquinone, it is colorless (Campbell et al. 2015). Therefore, the change of PCPIP’s color which is caused by the electron transport chain --- short circuits of light reaction can be measured by using a spectrophotometer set to 600 nm. High levels of photosynthesis in chloroplasts will decolonize DCPIP. DCPIP will be decolorized greater than less-active chloroplasts (Campbell et al. 2015). In this experiment, there were three types of samples including samples without chloroplasts in light, samples with chloroplasts in dark and samples with chloroplasts in light. The effort of light on the activity of photosynthesis was explored by measuring the color change of DCPIP (Campbell et al. 2015). A null hypothesis ( ) was that different light will have no effect on photosynthetic activity. An alternative hypothesis ( ) was made that different light will have an effect on photosynthetic activity. An experimental hypothesis was suggested that if there is an effect, in the samples which contain chloroplasts in light, the absorbance at 600 nm will decrease which means active photosynthesis will occur; in the samples which contain chloroplasts in dark and the samples without chloroplasts in light, the absorbance as 600 nm will maintain
2015). When DCPIP is oxidize d, it is blue; when DCPIP is reduced which means it snatches electrons from plastoquinone, it is colorless (Campbell et al. 2015). Therefore, the change of PCPIP’s color which is caused by the electron transport chain --- short circuits of light reaction can be measured by using a spectrophotometer set to 600 nm. High levels of photosynthesis in chloroplasts will decolonize DCPIP. DCPIP will be decolorized greater than less-active chloroplasts (Campbell et al. 2015). In this experiment, there were three types of samples including samples without chloroplasts in light, samples with chloroplasts in dark and samples with chloroplasts in light. The effort of light on the activity of photosynthesis was explored by measuring the color change of DCPIP (Campbell et al. 2015). A null hypothesis ( ) was that different light will have no effect on photosynthetic activity. An alternative hypothesis ( ) was made that different light will have an effect on photosynthetic activity. An experimental hypothesis was suggested that if there is an effect, in the samples which contain chloroplasts in light, the absorbance at 600 nm will decrease which means active photosynthesis will occur; in the samples which contain chloroplasts in dark and the samples without chloroplasts in light, the absorbance as 600 nm will maintain