Materials
1. Micropipette
2. Micropipette Tips
3. WARDS’S quick view DNA Stain
4. Prepared Agarose 0.8%
5. Tris-Borate-EDTA 5x Buffer
6. Electrophoresis Mold
7. Electrophoresis comb
8. Electrophoresis chamber
9. Electrophoresis power pack and cords
10. DNA from cow liver
11. DNA from chicken liver
12. 224 grams of fresh cow’s liver
13. 224 grams of fresh chicken liver
14. Meat tenderizer
15. Kitchen knife
16. Blender
17. 2.5 ml Salt
18. 31 ml dishwashing liquid (Dawn)
19. 354 ml warm water
20. Small test tube
21. Beaker
22. Cheesecloth
23. 354 cold isopropyl alcohol
24. Stirring rod
25. Microscope
26. Distilled water
27. Light Source
28. Clear plastic sheet
29. Plastic bowls
Procedures
Purification of the DNA
1. Using the kitchen knife, …show more content…
Place the pieces of liver into the blender.
3. Add the salt and warm water to the liver in the blender. Make sure the water covers the liver pieces.
4. Blend on high with the cover on for 10 to 15 seconds or unto blended completely.
5. Strain the blended mixture through cheesecloth into a …show more content…
Let sit for 20 minutes to harden.
7. Repeat as needed for each test.
Performing Gel Electrophoresis
1. Gather all materials needed. You will need electrophoresis chamber, power source, molds, Tris-Borate-EDTA 5x Buffer, DNA samples, and micro pipette.
2. Take the mold out of the mold plastic.
3. Place the gel mold in the electrophoresis chamber.
4. Using the Micro-Pipette fill the wells in the mold with the DNA sample.
5. Pour the buffer into the chamber ( do not pour directly onto the mold). Pour until it is about 1 to 1 ½ ml over the gel.
6. Plug in the power cables with the negative charge going on the side where the wells are.
7. Turn on the power source.
8. Let run for 35 minutes.
9. Turn the power source off and wait one minute for the last electrical current to run through than unplug.
10. Complete for each sample needed
Staining the molds.
1. Take the molds out of the electrophoresis chamber
2. Place the mold into a bowl be careful as the mold might slide.
3. Pour DNA stain into the bowl do not pour directly onto the mold.
4. Do this for each sample.
5. Stain for 24 hours.
Distaining the molds
1. Carefully take the molds out of the stain
2. Empty the stain out.
3. Pour warm (about 32 degrees Celsius) distilled water into the
1. Micropipette
2. Micropipette Tips
3. WARDS’S quick view DNA Stain
4. Prepared Agarose 0.8%
5. Tris-Borate-EDTA 5x Buffer
6. Electrophoresis Mold
7. Electrophoresis comb
8. Electrophoresis chamber
9. Electrophoresis power pack and cords
10. DNA from cow liver
11. DNA from chicken liver
12. 224 grams of fresh cow’s liver
13. 224 grams of fresh chicken liver
14. Meat tenderizer
15. Kitchen knife
16. Blender
17. 2.5 ml Salt
18. 31 ml dishwashing liquid (Dawn)
19. 354 ml warm water
20. Small test tube
21. Beaker
22. Cheesecloth
23. 354 cold isopropyl alcohol
24. Stirring rod
25. Microscope
26. Distilled water
27. Light Source
28. Clear plastic sheet
29. Plastic bowls
Procedures
Purification of the DNA
1. Using the kitchen knife, …show more content…
Place the pieces of liver into the blender.
3. Add the salt and warm water to the liver in the blender. Make sure the water covers the liver pieces.
4. Blend on high with the cover on for 10 to 15 seconds or unto blended completely.
5. Strain the blended mixture through cheesecloth into a …show more content…
Let sit for 20 minutes to harden.
7. Repeat as needed for each test.
Performing Gel Electrophoresis
1. Gather all materials needed. You will need electrophoresis chamber, power source, molds, Tris-Borate-EDTA 5x Buffer, DNA samples, and micro pipette.
2. Take the mold out of the mold plastic.
3. Place the gel mold in the electrophoresis chamber.
4. Using the Micro-Pipette fill the wells in the mold with the DNA sample.
5. Pour the buffer into the chamber ( do not pour directly onto the mold). Pour until it is about 1 to 1 ½ ml over the gel.
6. Plug in the power cables with the negative charge going on the side where the wells are.
7. Turn on the power source.
8. Let run for 35 minutes.
9. Turn the power source off and wait one minute for the last electrical current to run through than unplug.
10. Complete for each sample needed
Staining the molds.
1. Take the molds out of the electrophoresis chamber
2. Place the mold into a bowl be careful as the mold might slide.
3. Pour DNA stain into the bowl do not pour directly onto the mold.
4. Do this for each sample.
5. Stain for 24 hours.
Distaining the molds
1. Carefully take the molds out of the stain
2. Empty the stain out.
3. Pour warm (about 32 degrees Celsius) distilled water into the