Phenotype Fly Lab Report

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ABSTRACT In this experiment we set up crosses between mutant and wildtype flies in order to find similarities to Mendel’s work with pea plants. We did this by placing females in vials with males and waiting one week. After one week we obtained the second generation and were able to observe the different phenotypes that resulted from the cross. We were able to count each phenotype under a microscope and record the number of each type. We used this data to calculate the chi square value, which we compared to the table to determine whether or not the hypothesis was true.
INTRODUCTION
The law of segregation states that alleles of a trait separate independently during gamete formation such that each gamete receives only one allele from each allelic pair. Genetic traits are passed down from one generation to the next in different ways. For example, autosomal inheritance is a pattern in which transmission of the trait depends on
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We labeled two new vials in order to set up two new crosses. We placed three white-eyed females with three red-eyed males, and three red-eyed females with three white-eyed males. They were stored at 25° C for one week. During the second lab period, we anesthetized the parental flies and removed them to prevent further mating with the new generations. They were then stored at 25° C for another week. We anesthetized the flies and placed them onto a CO2 pad under a dissecting microscope. We then counted the number of males and females with each eye color. We placed three females and three males into a new vial that had been labeled. We completed these steps for both crosses. They were stored for another week at 25° C. On the fourth week, we retrieved the F1 intercrosses to anesthetize and remove the parental flies. They were stored for another week. We retrieved the flies and counted the number of each type of

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