For this experiment, unknown sample #12 was used. The first step in identifying any unknown bacterium is to grow a culture on a nutrient agar plate. This bacterium was also plated on a MAC plate, which is both selective for gram-negative bacteria and differential as well. On the NA plate, bright red colonies were present; on the MAC plate there was bacterial growth as well, but no color change was observed. This lack of color change on the MAC plate indicated that the bacteria did not ferment lactose.…
Abstract This experiment was a study of the different properties of a lipid bilayer. We used fluorescent microscopy to study the lateral diffusion of lipids and binding properties biotin and anti-biotin. The effect cholesterol has on the fluidity of the membrane was tested using Fluorescent Recovery After Photobleaching (FRAP). We used a Nikon Eclipse 50i upright fluorescent microscope with a Digital Sight DM-2MBW CCD camera to take images of the bilayer after photobleaching.…
Conversely, in Kligler iron agar media, which exams the ability of bacteria to ferment lactose and glucose, M.Luteus gave negative outcome which matches the expected result. Thus, I repeated the Kligler iron agar to test if M.Luteus can ferment glucose and I obtained same result which is negative. Getting positive result for glucose in beginning could be…
Red Dye 1 Red dye 1 is most likely a polar covalent molecule since we have found that it dissolves into water but does not break apart into ions. Just because it dissolves in water does not make it solely polar covalent, but since it has a relatively low conductivity, 534 mS/cm in the 0-20000 range, then it most likely is polar covalent. This is further confirmed with the fact that it does not react with NaOH, NaCl, or AgNO3, all ionic substances. The red dye does not react with NaOH since there was no color change when added, and the conductivity simple rose as the NaOH was added, due to the fact the excess ions were being added to the solution and not reaction at all.…
The Mixed Broth culture contained two unknown bacterial cells and they either Gram-negative or Gram-positive. The procedures were followed as stated from the course laboratory manual by Meramec Community College (1). The first step was figuring out the unknowns was to separate the two bacteria. In order to do this, the Trypticase Soy agar was used. The streaking method was used to spread the bacteria across the Trypticase Soy agar to isolate the bacteria.…
A positive result is positive growth of cells within it, and it shows that the bacteria inoculated within it is aerobic and growth is supported. A negative result is lack of growth and it indicates that the bacteria inoculated within it is not aerobic. The test works by being selective and only allowing the growth of aerobic bacteria. SF broth is a selective medium and is used to select certain organisms by providing the correct environment and nutrients needed to grow. A positive result will elicit a color change of the media from purple to yellow.…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…
The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder.…
Purpose: The overall goal of this lab was to perform a procedure on E. Coli which involved transferring genes that encoded for the green fluorescent protein into E. Coli to see if the transferred genes would make a difference on the growth and whether or not the bacteria would glow under UV light. Hypothesis: If the bacteria with the pGLO plasmid was grown on a plate containing LB and ampicillin then the bacteria will grow but not glow under UV light. If the bacteria with the pGLO plasmid was grown on a plate containing LB, ampicillin, and arabinose then it will be able to grow and glow under UV light. If the bacteria without pGLO plasmid was grown on a plate containing LB and ampicillin then it will not be able to grow or glow under UV light.…
Part B Two test tubes were labeled, one with “yeast” and the other with “water”. 1 ml of yeast suspension and 4 ml of distilled water was added to the “yeast’ labeled tube. 5 ml of distilled water was added to the “water” tube. 5 ml of sucrose solution was added to each test tube and stirred.…
MacConkey agar, which is media containing crystal violet and bile salts inhibits Gram positive bacteria (5). The media is also differential since the fermentation of lactose is detectable on MacConkey agar by pH indicators. Eosin Methylene Blue agar (EMB) contains chemicals that inhibit Gram positive growth and can identify lactose and sucrose fermentation through the previously described pH detection (3,5). Sometimes, media is developed to identify specific organisms. An example of this is Chromogenic…
The result indicated that the unknown #63 was positive for lactose fermentation and eliminated S. marcescens, P. mirabilis, and P. vulgaris, which are lactose nonfermenters. BCP (lactose) broth which was originally a purple colored medium turned into a color yellow and signs of gas production was also noted. The result indicated that the unknown #63 was positive for both acid and gas production and eliminated K. pneumoniae, and E. aerogenes because they can produce acid, but no gas production was suggested from the dichotomous key. The test result for SIM deep slant that was used to test for sulfur-reducing turned up positive due to its color changed into a blackening color. The test result eliminated P. aeruginosa and identified unknown #63 as being Citrobacter freundii.…
Each tube was labelled from one to six. 15 mL of solution with 10% concentration were added in the respective tube for starch, lactose, sucrose, glucose, fructose, and distilled water. 15 mL of distilled water and 15 mL 10% yeast suspension were poured to each fermentation tube as seen in Figure 1. All the mixtures were gently shaken at the same time without spilling the mixtures.…
Does a Measurement Result in a True Value? Limitations of Measurements Elizabeth Lechtholz-Zey & Marisa Loredo 10/5/15 CHEM 101-08 Purpose To determine the differences in precision and accuracy in weighing 10 mL of water using a 50 mL beaker, a 10 mL graduated cylinder, and a 50 mL buret. Data Temperature of water: 23.0ºC 100 mL beaker weight: 50.557 g # of additions of water to the 100 mL beaker * 50 mL beaker (±5 mL) * 10 mL graduated cylinder (±0.05 mL) * 50 mL buret (±0.05 mL) 0 0.00 g 0.00 g 0.00 g 1 7.48 g 9.91 g 9.95 g 2 14.23 g 19.70 g 19.83 g 3 21.38 g 29.56 g 29.85 g 4 29.20 g 39.44 g 39.77 g 5 35.91 g 49.30 g 49.72 g * 10 mL graduated cylinder (±0.05 mL) 0 50.557 g 1 60.340 g 2 70.010…
Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia.…