+ Pglo Plasmid Experiment

DNA plasmid was added to each of the “+plasmid” and it expresses the ampicillin resistance gene. According to the data, that didn’t happen because the +pGLO LB/amp had 3 white colonies at the end of the bacterial transformation and the +pGLO LB/amp/ara had 2 colonies that glow at the end of bacterial transformation meaning that there was an error in our “+plasmid” which occurred during the lab. We did predict that the -pGLO LB/amp would remain the same with no colonies and -pGLO LB would grow bacteria because the “-plasmid” does not express the ampicillin resistance gene. According to the data, The -pGLO LB/amp had no colonies present at the end of its transformation and the -pGLO LB had bacteria present at the end of its transformation which …show more content…
According to the results, The +pGLO LB/amp/ara had 2 colonies that glowed when UV light was put on them, this shows that transformation has taken place. Also, for the “+plasmids” there was suppose to be an increase in bacterial colonies due to the ampicillin resistance gene and plasmid DNA but the number of colonies that were present in this procedure were 2 bacterial colonies for the +pGLO LB/amp/ara and 3 colonies for the +pGLO LB/amp meaning that there must have been an error that took place in the …show more content…
According to the results, The +pGLO LB/amp/ara had 2 colonies that glowed when UV light was put upon them, which shows that transformation has taken place and the +pGLO LB/amp had 3 white colonies at the end of its bacterial transformation. In reality the “+plasmid” plates were supposed to contain more colonies that were present due to the ampicillin resistant gene. The -pGLO LB/amp had no colonies present at the end of its transformation and the -pGLO LB had bacteria present at the end of its transformation which shows that it had a successful transformation that took place unlike the “+plasmid” plates which didn't have as much of a successful transformation that took