An inverse relation between peroxidase activity and ascorbate content suggests the involvement of ascorbate-peroxidase system in the increased secretion of progesterone resulting from LH action in rabbit. Also the same system renders some evidence for the involvement of this enzyme along with other factors in the process of implantation.
Key Words: Reproductive tract, Peroxidase-Ascorbate system Pregnancy & Implantation
Introduction:
It has been reported that the LH in various species of animals stimulates progesterone biosynthesis or secretion by the luteal tissue or the luteinized ovary (Savard et al., 1965; Amstrong, 1968). Injection of LH to rabbits increased progesterone,20α-OH-progesterone and estrogen in ovarian venous plasma (Eaton …show more content…
(1951) after proceeding for calibration of caesin).
Ascorbic Acid:
Ascorbate was determined by the colorimetric method of Mindlin and Butler (1938) by following the decolorization of 2,6 dichlorophenolindophenol in metaphosphoric acid after proceeding for calibration of Ascorbic Acid.
Peroxidase Activity: Total peroxidase activity was measured using guaiacol as donor by the method of Maehly and Chance (1954).
Results: Uterus Fig. 9c : Changes in ascorbate and peroxidase activity in uterus during different days of pregnancy in rabbit During the first 4 days of pregnancy when the blastocyst is inside the fallopian tube the uterus shows weak peroxidase activity and the AA content is high (Fig.9c). While the blastocysts were still free in the uterine lumen or had just begun to make endometrial contact, a sharp rise in peroxidase activity is seen in the uterus at day 5 and 7 which is maintained till day 10 and ascorbate content remains at a low steady …show more content…
The results presented are in agreement with that of luteal activity of the ovary and progesterone secretion during the early period of pregnancy in rats charaterised by the presence of high peroxidase activity and depletion of ascorbate (Agrawal and Laloraya, 1979). The presence of high peroxidase activity in the fallopian tube containing the blastocyst and in the uterus after day 5, when the blastocyst is about /ready to implant, suggests that peroxidase may also be involved in regulating the adhesiveness of the deciduoma for the attachment of the blasotcyst as suggested earlier (Agrawal and Laloraya,1979). Peroxidase was suggested to have a role in regulating the stickiness of the uterine endometrium and blastocyst or of both, which is essential for implantation (Agrawal &
Key Words: Reproductive tract, Peroxidase-Ascorbate system Pregnancy & Implantation
Introduction:
It has been reported that the LH in various species of animals stimulates progesterone biosynthesis or secretion by the luteal tissue or the luteinized ovary (Savard et al., 1965; Amstrong, 1968). Injection of LH to rabbits increased progesterone,20α-OH-progesterone and estrogen in ovarian venous plasma (Eaton …show more content…
(1951) after proceeding for calibration of caesin).
Ascorbic Acid:
Ascorbate was determined by the colorimetric method of Mindlin and Butler (1938) by following the decolorization of 2,6 dichlorophenolindophenol in metaphosphoric acid after proceeding for calibration of Ascorbic Acid.
Peroxidase Activity: Total peroxidase activity was measured using guaiacol as donor by the method of Maehly and Chance (1954).
Results: Uterus Fig. 9c : Changes in ascorbate and peroxidase activity in uterus during different days of pregnancy in rabbit During the first 4 days of pregnancy when the blastocyst is inside the fallopian tube the uterus shows weak peroxidase activity and the AA content is high (Fig.9c). While the blastocysts were still free in the uterine lumen or had just begun to make endometrial contact, a sharp rise in peroxidase activity is seen in the uterus at day 5 and 7 which is maintained till day 10 and ascorbate content remains at a low steady …show more content…
The results presented are in agreement with that of luteal activity of the ovary and progesterone secretion during the early period of pregnancy in rats charaterised by the presence of high peroxidase activity and depletion of ascorbate (Agrawal and Laloraya, 1979). The presence of high peroxidase activity in the fallopian tube containing the blastocyst and in the uterus after day 5, when the blastocyst is about /ready to implant, suggests that peroxidase may also be involved in regulating the adhesiveness of the deciduoma for the attachment of the blasotcyst as suggested earlier (Agrawal and Laloraya,1979). Peroxidase was suggested to have a role in regulating the stickiness of the uterine endometrium and blastocyst or of both, which is essential for implantation (Agrawal &