P53 And Its Pro Apoptotic And Pro Oxidant Downstream Target Genes

888 Words Nov 27th, 2015 4 Pages
In the present study we showed that, the NAFLD results in a significant elevation in p53 and casapase III expression and down-regulates the bcl-2 expression and biosynthesis. Biochemical analyses showed that CMN-extract in simultaneous form of administration with UDCA remarkably diminishes the NAFLD-induced pathological effects in the liver of examined animals. In order to find other possible associated mechanism(s) with apoptosis, the tissue antioxidant status, NO levels as well as serum biochemical factors were analyzed. It was shown that co-administration of CMN-extract and UDCA significantly improved the antioxidant power of tissue by up-regulating the enzymatic antioxidant status and reducing the inflammatory reactions. Reduced tissue NO and iNOS levels as well as ameliorated serum biomarkers confirmed our other findings. The importance of p53 and its pro-apoptotic and pro-oxidant downstream target genes in ALFD and NAFLD is highlighted by the reports showing genetic ablation of p53 significantly reduced both diseases-induced hepatocellular injury and oxidative stress [45-47]. On the other hand, it has been showed that p53 regulates lipid accumulation in hepatocytes by directly binding to the G6PD that in turn results in a significant inhibition in pentose phosphate pathway [49]. Our IHC and RT-PCR results showed that the expression and/or biosynthesis of p53 were significantly higher in non-treated NAFLD-induced animals. Meanwhile, co-administration of CMN-extract with…

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