Abstract
The primary goal for this laboratory experiment was to synthesize, purify, and identify p-nitroacetanilide using acetanilide as the starting material. To test for the purity and identification of our desired product, we gathered data from: Thin Layer Chromatography (TLC), Melting Point (MP) range, Infrared (IR) spectrum, Gas Chromatography Mass Spectroscopy (GC-MS), and Proton Nuclear Magnetic Resonance (1H NMR). TLC plate indicated the formation of our pure product due to the difference in Rf values between starting material and product, 0.83 and 0.72 respectively. IR spectrum showed a peak at 3274 m-1, indicating the presence of N-H stretch for p-nitroacetanilide; whereas, the GC-MS results showed a peak at retention time 10.11 minutes with a value of 180.1 m/z, which correlated to …show more content…
Specifically, nitration plays an important role in regulation of key enzyme activities and signal transduction networks. Tyrosine is one of the 21 amino acids and is important in the synthesis of certain hormones. Nitration of tyrosine can affect protein function, which can in turn link nitroxidative stress to alterations of molecules present in disease (Peluffo et al. 2007). Potential activation of proteins, specifically tyrosine, can have a significant impact on the pathology of cardiovascular disease. Because of this, nitrotyrosine is being recognized as a crucial independent marker of cardiovascular disease (Peluffo et al. 2007). Our primary goal for this experiment was to synthesize, purify, and identify p-nitroacetanilide. First, we performed a series of chemical reactions to yield our desired product. Then, to test for purity and to furthermore scrutinize the molecular of our desired product, we used Gas Chromatography-Mass Spectroscopy (GC-MS), Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy, Infrared Spectroscopy (IR), Thin Layer Chromatography (TLC), and the Melting Point (MP) range.
The primary goal for this laboratory experiment was to synthesize, purify, and identify p-nitroacetanilide using acetanilide as the starting material. To test for the purity and identification of our desired product, we gathered data from: Thin Layer Chromatography (TLC), Melting Point (MP) range, Infrared (IR) spectrum, Gas Chromatography Mass Spectroscopy (GC-MS), and Proton Nuclear Magnetic Resonance (1H NMR). TLC plate indicated the formation of our pure product due to the difference in Rf values between starting material and product, 0.83 and 0.72 respectively. IR spectrum showed a peak at 3274 m-1, indicating the presence of N-H stretch for p-nitroacetanilide; whereas, the GC-MS results showed a peak at retention time 10.11 minutes with a value of 180.1 m/z, which correlated to …show more content…
Specifically, nitration plays an important role in regulation of key enzyme activities and signal transduction networks. Tyrosine is one of the 21 amino acids and is important in the synthesis of certain hormones. Nitration of tyrosine can affect protein function, which can in turn link nitroxidative stress to alterations of molecules present in disease (Peluffo et al. 2007). Potential activation of proteins, specifically tyrosine, can have a significant impact on the pathology of cardiovascular disease. Because of this, nitrotyrosine is being recognized as a crucial independent marker of cardiovascular disease (Peluffo et al. 2007). Our primary goal for this experiment was to synthesize, purify, and identify p-nitroacetanilide. First, we performed a series of chemical reactions to yield our desired product. Then, to test for purity and to furthermore scrutinize the molecular of our desired product, we used Gas Chromatography-Mass Spectroscopy (GC-MS), Proton Nuclear Magnetic Resonance (1H NMR) spectroscopy, Infrared Spectroscopy (IR), Thin Layer Chromatography (TLC), and the Melting Point (MP) range.