Osmosis And Haemolysis Lab Report

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In this experiment we aim to that % hemolysis is going to decrease as osmolality increases because the more concentrate is the solution, the less cells going through hemolysis. Thus, there is not enough water entering the cell to produces the cells to burst. The main clue is when the water is completely dilute, so we have 100% hemolysis. Hemolysis is correlated with the behavior of the cell specifically refers as hypotonic.
The results correlated with the raised hypothesis, the data overall represents the hypothesis. The different osmolality has a relationship with the % hemolysis. Starting with the distilled water which complete hemolysis was obtained. When we obtained the highest osmolality, the % hemolysis decreases to zero proving that
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Goodhead and Frances M. MacMilla, osmosis is a process that is fundamental for life where water and small molecules cross the semipermeable membrane. They hypothesized that being in a hypotonic solution can lead to hemolysis. Their experiment called “Measuring osmosis and hemolysis of red blood cells” proved that when the concentration is low, and there is high network of water going inside the cell, then it produces the cells to increase the amount of cells breaking or going through hemolysis. According to the researchers, “No change in cell volume occurs in isotonic NaCl, and, by placing blood cells in hypotonic NaCl, incomplete hemolysis occurs. By changing the bathing solution to either distilled water or isosmotic urea, complete hemolysis occurs due to their hypotonic effects.” As a result this demonstrated again how the response of cells (bursting) has a closed relationship with the concentration (osmolality). On this experiment, researchers obtained the same trend as on our experiment. For Lauren K. Goodhead and Frances M. MacMillan’s experiment, in distilled water, the result was 100% hemolysis compared with 0.45 mOsm/kg which % hemolysis was zero.
According to the author of the article “Cell Biology OLM” Stephen Gallik, the % of red blood cells that go through hemolysis depended on the hypotonicity of the fluid. They used a graph to demonstrate that hemolysis does not occur when solution is above 0.6% NaCL, but until the NaCL

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