Temperatures have a great effect on microorganisms. In the most general sense, bacteria can grow at any temperature between freezing point of water (32°C) and the point where proteins coagulate. Between these two high and low temperatures, each bacteria type has an optimum growth temperature. An optimal growth temperature is the point at which an organism grows best, with optimal cell metabolism. If temperatures creep past the maximum, bacteria are killed. If the temperature drops below the minimum, bacterial growth slows or stops but the organisms do not die. In regards to growth curve and generation time, temperature is a major factor as well. Because temperatures below the optimal temperature of an organism do not kill the organism, just
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coli is 37 oC. The temperature with the least amount of growth for E. coli is 65 oC. This is because it is past the maximum temperature, and therefore the bacteria started dying. The optimal temperature for B. stearothermophilius is 65 oC. The temperature with the least amount of growth for B. stearothermophilius is 25 oC. Because B. stearothermophilius is a thermophile, it likes to be grown in high temperatures and cannot be killed with excessive heat. Therefore, the lowest temperature had the least amount of growth because it slowed growth the most. E. coli had the longest generation time of 75 minutes. B. stearothermophilius had a shorter generation time of 60 minutes. A shorter generation time means that the bacterium can double its population in less time.
It is important to keep bacteria at its correct incubation temperature in order to keep them alive. If a person would like to study bacteria, they need to have viable cells to study. It is also important to know the temperature that will result in the fastest growth, in order to get the most visible growth (4). Cell density is the number of cells found in one area. Optical density (O.D.) is the same as absorbance, and it is used for measuring bacterial growth, which would be the number of cells
coli is 37 oC. The temperature with the least amount of growth for E. coli is 65 oC. This is because it is past the maximum temperature, and therefore the bacteria started dying. The optimal temperature for B. stearothermophilius is 65 oC. The temperature with the least amount of growth for B. stearothermophilius is 25 oC. Because B. stearothermophilius is a thermophile, it likes to be grown in high temperatures and cannot be killed with excessive heat. Therefore, the lowest temperature had the least amount of growth because it slowed growth the most. E. coli had the longest generation time of 75 minutes. B. stearothermophilius had a shorter generation time of 60 minutes. A shorter generation time means that the bacterium can double its population in less time.
It is important to keep bacteria at its correct incubation temperature in order to keep them alive. If a person would like to study bacteria, they need to have viable cells to study. It is also important to know the temperature that will result in the fastest growth, in order to get the most visible growth (4). Cell density is the number of cells found in one area. Optical density (O.D.) is the same as absorbance, and it is used for measuring bacterial growth, which would be the number of cells