Optimization of Gas Chromatography Essay

1543 Words Oct 21st, 2013 7 Pages
CHM 510 LABORATORY REPORT

Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature

Name: AFIQ B. ANWAR
Student No.: 2012621072 (AS2253A)
Date of experiment:
Date of report submission:
Lecturer’s Name: PN. HALIZA

Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature
INTRODUCTION
The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds. On top of that, the experiment is conducted to explore the gas chromatography, which is include the concepts of retention time and resolution using a mixture of methyl esters: methyl linolate, methyl laurate, methyl myristate, and
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Preparation of separation of methyl esters using column temperature programming
1) The standard was injected at the optimal carrier gas flow rate using a linear temperature ramp was injected from 100 to 290 at 70cm/s.
Preparation to identification of components in methyl ester mixture
1) Each methyl ester individually was injected to identify the various compounds in the standard mixture using the optimized GC condition.
2) The result is recorded.

RESULT
Individual standard:
Optimum Temperature & flowrate (210 ͦ C, 70cm/s)
Compound
Retention time (min)
Width (min)
Peak area
Methyl Laurate
1.078
0.0191
835.686
Methyl Myristate
1.461
0.0298
5509.449
Methyl Palmitate
2.217
0.0538
219.353

Standard Mixture
1. 210 and 70cm/s
Compound
Retention time
Width
Area
Mobile solvent
0.720
0.0111
18750.1
Methyl Laurate
1.071
0.0182
412.605
Methyl Myristate
1.446
0.0288
1801.308
Methyl Palmitate
2.206
0.0523
3132.187

2. 210 and 50cm/s
Compound
Retention time
Width
Area
Mobile solvent
1.021
0.0126
154681.5
Methyl Laurate
1.512
0.0218
378.878
Methyl Myristate
2.037
0.0357
1674.902
Methyl Palmitate
3.102
0.0651
2939.639

3. 210 and 30cm/s
Compound
Retention time
Width
Area
Mobile solvent
1.714
0.0211
194772.5
Methyl Laurate
2.532
0.0334
457.218
Methyl Myristate
3.405
0.0520
1965.931
Methyl Palmitate
5.179
0.0904

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