Nutrient Agar Lab Report

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The antimicrobial activity of QTR-CS were determined using the agar well diffusion method. Nutrient agar medium was prepared, autoclaved at 120°C for 15 minutes. Then in the sterile condition the medium was poured into the plates and allowed to solidify. Bacterial suspension was prepared by taking a loopful of cultures of gram positive and gram negative bacteria later dissolved in 1ml of sterile distilled water separately for each culture. 0.1ml of the bacterial suspension was placed on the nutrient agar medium and spread using a sterile swab. Wells of 6 mm diameter were made in the solidified media with the help of sterile borer followed by addition of 50µl of the samples into each well. The plates were incubated at 37°C for 24 hours and

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