The effect of NS crude oil pretreatment on tumor volume
Analysis of the data on tumor volume showed that a 4-month pretreatment with NS crude oil at the dose of 4 ml/kg/day results in significantly reduced volumes (550.46mm3±48.05; n=7) when compared to the control group (875.46.5mm3±80.17).
NS1 tumor volumes (1322.83mm3±128.8; n=7) were significantly larger than the control volumes (Figure 1). There were no significant differences in mean volume of tumor tissue between NS2 and control group.
The effect of NS crude oil pretreatment on antioxidant enzymes activity
Both SOD and CAT activities, at the dose of 4 ml/kg/day of NS crude oil, were significantly higher than those of the control group with P<0.05 (n=4) (Figure 2, 3).
In comparison with controls, no …show more content…
The differences in VEGF levels between NS2 and Control groups did not reach to statistical significance (Figure 4).
The effect of NS crude oil pretreatment on endostatin levels
Endostatin amount was significantly higher than the control group at the dose of 4 ml/kg/day (n=4) but at the dose of 1 ml/kg/day of NS crude oil there was a significant reduction in endostatin levels (n=4) (Figure 5). The differences in endostatin levels between 2 ml/kg/day and control groups did not reach to statistical significance.
Discussion
In the present study, we demonstrated that the mice pretreated with 4 ml/kg/day of NS oil had significantly smaller tumor volumes, higher SOD and CAT activity, reduced VEGF and increased endostatin amounts. We believe that Nigella sativa crude oil, hence, seems to inhibit breast tumor growth in part by improving the activity of antioxidant enzymes and in part by interfering with angiogenesis, although more studies are, yet, required to better illuminate its other mechanisms of
Analysis of the data on tumor volume showed that a 4-month pretreatment with NS crude oil at the dose of 4 ml/kg/day results in significantly reduced volumes (550.46mm3±48.05; n=7) when compared to the control group (875.46.5mm3±80.17).
NS1 tumor volumes (1322.83mm3±128.8; n=7) were significantly larger than the control volumes (Figure 1). There were no significant differences in mean volume of tumor tissue between NS2 and control group.
The effect of NS crude oil pretreatment on antioxidant enzymes activity
Both SOD and CAT activities, at the dose of 4 ml/kg/day of NS crude oil, were significantly higher than those of the control group with P<0.05 (n=4) (Figure 2, 3).
In comparison with controls, no …show more content…
The differences in VEGF levels between NS2 and Control groups did not reach to statistical significance (Figure 4).
The effect of NS crude oil pretreatment on endostatin levels
Endostatin amount was significantly higher than the control group at the dose of 4 ml/kg/day (n=4) but at the dose of 1 ml/kg/day of NS crude oil there was a significant reduction in endostatin levels (n=4) (Figure 5). The differences in endostatin levels between 2 ml/kg/day and control groups did not reach to statistical significance.
Discussion
In the present study, we demonstrated that the mice pretreated with 4 ml/kg/day of NS oil had significantly smaller tumor volumes, higher SOD and CAT activity, reduced VEGF and increased endostatin amounts. We believe that Nigella sativa crude oil, hence, seems to inhibit breast tumor growth in part by improving the activity of antioxidant enzymes and in part by interfering with angiogenesis, although more studies are, yet, required to better illuminate its other mechanisms of