Nitrogen Cycle Essay

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Nitrogen cycling is a vital factor in how plants and organisms survive in ecosystems. Different genes in bacteria DNA allows for NO₃, in plants, to be converted to N₂ without releasing N₂O into the atmosphere. With a better understanding of which gene can convert N₂O to N₂, people can have a better understanding of what factors affect the nitrogen cycle. The genes that are responsible for converting NO₃ to N₂ are nirk, cnor, qnor, and nosz. Nosz is responsible for coding enzymes to convert N₂O to N₂ Using a DNA Isolation Kit, we were able to take DNA from the microbial community and amplified the specific genes using a PCR system. From the PCR, we were able to find the results of the presence of the four genes of bacteria at different soil …show more content…
After amplifying the specific genes from microbial DNA at different sites, we found that nosz was more present in soils under aerobic conditions than under anaerobic conditions. This could be due to the fact that there is less nitrogen in water than in soil. Introduction: Nitrogen cycling allows for living organisms to survive in ecosystems. The nitrogen cycle consists of N₂ from the atmosphere being changed and converted to NO₃ for organisms to properly and then back to N₂. This process of converting N₂ to NO₃ is called nitrogen fixation. First, bacteria microbes will convert N₂ to NH₃ and then to NH₄. This part of nitrogen fixation is known as ammonification. The NH₄ is then converted to NO₂ and then to NO₃. This part of nitrogen fixation is known as nitrification. After this process, plants can safely absorb the …show more content…
The tube was then mixed by gently being vortexed. 60 µl of not precipitated solution C1 was then added to the tube and vortexed briefly. The tube was then secured horizontally on flat-bed vortex pad and vortexed at maximum speed for 10 minutes. After 10 minutes, the tube was put into a centrifuge and spun at 10,000 x g for 30 seconds at room temperature. After the tube was centrifuged, the solution from the PowerBead tube was transferred to a 2 ml collection tube. 250 µl of solution C2 was added to the tube and the ube was vortexed for 5 seconds. After 5 seconds, the tube was incubated at 4 ℃ for 5 minutes. After 5 minutes, the tube was centrifuged at room temperature for 1 minute. 600 µl of the solution in the tube was then transferred to a clean 2 ml tube. Once transferred, 200 µl of solution C3 was added to the tube. The tube was then vortexed briefly and was incubated at 4 ℃ for 5 minutes. After incubation, the tube was centrifuged at room temperature for 1 minute. After the centrifuge, 750 µl of the solution was transferred to a clean 2 ml tube. Once transferred, 1.2 ml of solution C4, after being shaken and mixed, was added to the tube. The tube was then vortexed for 5 seconds. After that, 675 µl of solution was loaded onto a spin filter and centrifuged for 1 minute. After discarding the flow through, another 675 µl of solution was added to the spin filter. The spin filter was then

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