There are many ligands that made up the chemokine receptor CXCR3 for the successful mediation of gene transcription. These ligands include the following CXCL 11, CXCL 10, and CXCL 9. DNA purification (Mini prep) helps to determine the concentration of both GFP and NFkB DNA before being used to transfect both HeLa and HEK 293 cell. The purification of DNA also allows the extraction and purification of plasmid DNA. According to the data that was generated by the completion of the Mini Prep, GFP DNA tends to have a higher concentration of 27.544 µg compared to NFkB DNA that has 16.950 µg. GFP DNA is considered to have properly purified because it yields a high number of concentration at the end of this process. GFP DNA successfully transfected
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Figure 7 shows induced NFkB activation in HeLa cells by the stimulant TNF alpha present in a bar chart. TNF alpha uses receptor to make sure the NFkB activation works well in the cell. Several reporter systems have been used to study the expression and activation of NFkB. In reference to the bar chart to figure 7, Clone AH and Clone GR tends to have the highest value of TNF alpha by the completion of stimulation while Clone KS and Clone BM show the lower values of TNF alpha considering their bar charts that was displayed above. Clone KS has the lowest unstimulated value compared to clone GR that has the highest unstimulated value. Clone GR has the highest TNF alpha value compared to clone KS that exhibit the lowest TNF alpha value. Also, Figure 8 shows another bar chart of HeLa cell that was stimulated with TNF alpha induced NFkB activation. The differences between stimulated (20ng/ml of TNF alpha) and unstimulated HeLa cell as a result of the luciferase assay …show more content…
Clone AH tends to exhibit the highest value of both Unstimulated and stimulated Hela cells while clone CU has the lowest of both unstimulated and stimulated HeLa cell value. Figure 9 displays different stimulant in both HeLa and Hek293 cell TNF alpha induced NFkB activation. In this case, Hela cell and Hek293 cell both stimulated with different sets of stimulant and the bar chart shows the distinct different after undergoing the luciferase activity. The bar charts clearly explain which of the two cells was properly stimulated by comparing the cells that has the higher value while being with the same stimulant. According to the above bar chart, SAA tends to have the highest value when being stimulated with Hek293/CXCR3 cell while TNF alpha shows a bit high value that stimulated HeLa/CXCR3 cell. An important role of CXCR3 and its ligands in the in this NFkb Luciferase reporter activity assay is the migration of T lymphocytes stems from adhesion experiments under flow conditions which fasten chemokine receptor CXCR3 to mediate gene
Figure 7 shows induced NFkB activation in HeLa cells by the stimulant TNF alpha present in a bar chart. TNF alpha uses receptor to make sure the NFkB activation works well in the cell. Several reporter systems have been used to study the expression and activation of NFkB. In reference to the bar chart to figure 7, Clone AH and Clone GR tends to have the highest value of TNF alpha by the completion of stimulation while Clone KS and Clone BM show the lower values of TNF alpha considering their bar charts that was displayed above. Clone KS has the lowest unstimulated value compared to clone GR that has the highest unstimulated value. Clone GR has the highest TNF alpha value compared to clone KS that exhibit the lowest TNF alpha value. Also, Figure 8 shows another bar chart of HeLa cell that was stimulated with TNF alpha induced NFkB activation. The differences between stimulated (20ng/ml of TNF alpha) and unstimulated HeLa cell as a result of the luciferase assay …show more content…
Clone AH tends to exhibit the highest value of both Unstimulated and stimulated Hela cells while clone CU has the lowest of both unstimulated and stimulated HeLa cell value. Figure 9 displays different stimulant in both HeLa and Hek293 cell TNF alpha induced NFkB activation. In this case, Hela cell and Hek293 cell both stimulated with different sets of stimulant and the bar chart shows the distinct different after undergoing the luciferase activity. The bar charts clearly explain which of the two cells was properly stimulated by comparing the cells that has the higher value while being with the same stimulant. According to the above bar chart, SAA tends to have the highest value when being stimulated with Hek293/CXCR3 cell while TNF alpha shows a bit high value that stimulated HeLa/CXCR3 cell. An important role of CXCR3 and its ligands in the in this NFkb Luciferase reporter activity assay is the migration of T lymphocytes stems from adhesion experiments under flow conditions which fasten chemokine receptor CXCR3 to mediate gene