Detection Of Neisseria Gonorrhoeae

Improved Essays
Diagnosis

The most appropriate test to detect Neisseria gonorrhoeae is the nucleic acid amplification test, also know as NAAT. NAAT consist of a urine sample, endocervical and vaginal swab for women, and a urethral swab in men (CDC, 2015). Test detects the genetic material of the bacteria, in this case N. gonorrhoeae. Theoretically the test is able to detect a single copy of the nucleic acid of the bacteria (AACC, 2014). The test amplifies the number of copies of the genetic material in order for the bacteria to be identified. One of the ways microbiologist amplify nucleic acid is the polymerase chain reaction (PCR); It is based on the DNA polymerase to synthesize a new strand of DNA that is complementary to the template strand provided. There is also the ligase chain reaction (LCR). The nucleic acid is used as the probe. Two probes are used per each DNA strand and are ligated together to form one
…show more content…
Furthermore, because of this issue a culture is also needed to detect resistance to antibiotics. For a culture test a endocervical or urethral swab specimen is needed. Specimen can also be taken from the rectum and pharyngeal. The specimen is streaked onto a Thayer-Martin or Martin Lewis selective medium. It can also be streaked onto a chocolate agar nonselective medium and incubated at thirty-five Celsius to thirty-six Celsius and with a five percent of carbon dioxide. A gram stain and oxidase test is then preformed and positive results will show a gram negative, oxidase positive diplococcic bacteria. (Johnson, 2002). Chlamydia trachomatis is a disease that has similarities to N. gonorrhoeae. Both infections are spread through a strain of bacteria that is spread through sexual contact. Both infection can be spread through the baby during birth. Also, both chlamydia and gonorrhea might not show any outward visible symptoms. The procedure for testing for either infections is the same and in most cases will be treated with the same type of medication (Chico,

Related Documents

  • Superior Essays

    Gram Catalase Test Report

    • 1163 Words
    • 5 Pages

    Abstract: The objective of these series of experiments was to identify two unknown bacteria’s. Broth culture #20 was selected and subjected to qualitative tests for identification. Gram stain tests were performed in order to identify which unknown is gram positive and gram negative. Using selective and differential media, like MacConkey agar which allows the growth of gram negative bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects gram-positive bacteria.…

    • 1163 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    We will be adding a couple of drops of Kovacs reagent following the incubation of the unknown organism. The indole combines with the reagent and if positive it produces a red layer on top of the medium. If the medium has a hazy growth throughout, then is positive for motility and if it produces Hydrogen Sulfide (H2S) a black precipitate is produced. Some bacteria can produce large amounts of stable acids and others produce a smaller amount. In order to know which ones produce more or less we will do the Methyl Red Test (MR) yellow cap tube.…

    • 1262 Words
    • 6 Pages
    Improved Essays
  • Great Essays

    Mannitol Salt Agar

    • 1936 Words
    • 8 Pages

    The first test involved the Mannitol Salt Agar (MSA). MSA is an example of both a selective and a differential media. It selects for halotolerant bacteria and with the aid of phenol-red differentiates for the ability of a microbe to ferment mannitol or not. If a microbe has the ability to ferment mannitol, it produces acidic waste products which react with the phenol-red to yield a yellow color. If the microbe is unable to ferment mannitol, it will consume the proteins present and will produce a pink/red color.…

    • 1936 Words
    • 8 Pages
    Great Essays
  • Improved Essays

    Lac Operons

    • 1587 Words
    • 6 Pages

    Genetic mutations are the source of numerous hereditary diseases. This has been discovered by the comparison of two DNA sequences with bioformatic software. Two sequences are aligned and compared in order to locate mutations in the DNA (Module 4 Protocol,1). Escherichia coli aka E. coli was used as the test subject in this lab because it employs lac operons while transcribing mRNA. An operon is a unit of linked genes that regulates genes in charge of protein synthesis.…

    • 1587 Words
    • 6 Pages
    Improved Essays
  • Improved Essays

    is suspected as the cause so they will choose the correct testing media (selective agar media such as SS agar plates) to distinguish Salmonella from other potential bacterial pathogens such as E. coli, Shigella strains, Staphylococcus food poisoning, or from toxins like botulism or pesticides. The majority of Salmonella isolates come from the feces of the infected person. Occasionally, Salmonella can be cultured from blood samples. Serovars are identified by serotyping (detecting bacterial proteins by using specific immunological tests). Definitive diagnosis usually requires that the bacteria be isolated and identified by these techniques.…

    • 797 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Unknown Lab Report

    • 1511 Words
    • 6 Pages

    Determining the cell shapes will help with the process of elimination organisms. Under microscopic examination for my unknown 16 broth, it appears to be a coccus shape and a gram positive. Gram staining can determine cell morphology, size, and arrangement. In a positive gram stain, a primary stain called crystal violet is used to stain the cell. For every new stain, mordant, decolorizing agent, and counterstain, it must be rinsed with distilled water.…

    • 1511 Words
    • 6 Pages
    Improved Essays
  • Improved Essays

    The16S rRNA primers used in this PCR, are complimentary to sequences conserved in all bacteria and will target sites of variation. These sites of variation were the regions of the DNA amplified by PCR. The products of PCR cannot be sequenced correctly without purification. Purification removes left over reactants and enzymes. A spin column-collection tube complex is used to collect the DNA during a series of centrifugation techniques with different buffers.…

    • 1466 Words
    • 6 Pages
    Improved Essays
  • Great Essays

    Thus, this bacteria can be stored for a months. The DNA isolate is important in study the genetic cause of disease and for the development of diagnostic and drugs. It is also essential in forensic science, sequencing genomes,determine paternity and detecting of microbe in the environment.DNA from the isolate bacteria is used in PCR. PCR is a powerful amplification technique that generate many an ample supply of a specific segment at DNA or designed to amplify the 16S RNA gene. There is two specific PCR primer were designed to amplify gene in expected size of 1500bp of a consensus 16S rRNA: forward primer, 8f and reverse primer,U1492R.…

    • 1716 Words
    • 7 Pages
    Great Essays
  • Improved Essays

    MSA also contains mannitol, which allows differential identification because an indicator detects acid produced by the fermentation of mannitol (4). MacConkey agar, which is media containing crystal violet and bile salts inhibits Gram positive bacteria (5). The media is also differential since the fermentation of lactose is detectable on MacConkey agar by pH indicators. Eosin Methylene Blue agar (EMB) contains chemicals that inhibit Gram positive growth and can identify lactose and sucrose fermentation through the previously described pH detection (3,5). Sometimes, media is developed to identify specific organisms.…

    • 1018 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Later some Elution Buffer was used to separate the DNA from the membrane of the spin column. Day two consisted of making the plasmid DNA resistant to Kanamycin and Ampicillin. Day three we made 0.8% agarose gel and ran electrophoresis to be able to see the weight of the DNA. Day four we genetically transferred the ligated plasmid DNA to E.coli and plated the cell mixture. Day five we looked at our results and went over them.…

    • 1536 Words
    • 6 Pages
    Improved Essays

Related Topics