Figure 1a. Size of nano curcumin. Image of curcumin nanoparticles taken with Transmission electron microscope (JOEL 2100F) showing particles having size distribution of 59.9 nm to 198 nm ( average size of 160 nm).
Figure 1b. Bioavailability of nano curcumin. Mice were given I.P injections with curcumin/nanocurcumin (vehicle: PBS containing 10% ethanol) per mice at 100 mg/kg body weight and then bled at different time points via retro-orbital puncture and blood collected from each mouse and subjected to analysis by HPLC.
Figure 2. Lung and spleen CFU. Mice infected with a low-dose aerosol inoculum (~150 CFU/mice) of M.tb strain H37Rv and treatment of nano curcumin started 7 days post infection. Mice were sacrificed at various …show more content…
Inhibition of Tem gives an opportunity to generate more Tcm, which will enable better vaccine efficacy. Kv1.3 is preferentially expressed in effector memory T cells (Tem), and hence this can be targeted for the inhibition of Tem. A well-known inhibitor for Kv1.3 is clofazamine, which in used for several indications including TB. Therefore, we would like to test if addition of clofazamine during vaccination improves vaccine efficacy of BCG. Although clofazimine is an approved drug for several indications, it is not expectedly safe. It has a half life more than 28 days, and accumulates in different organs (19). Curcumin is also inhibitor for Kv1.3, however its bioavailability is poor and it failed in phase I clinical trials (20). Recently, we developed nano-curcumin which has longer bioavailability, and is more efficacious for TB therapy. Therefore, we would like to test if addition of nano-curcumin enhances BCG vaccine efficacy. Although, BCG is not an effective vaccine it’s being used since its inception. Therefore, a large number of people are already vaccinated. The vaccinated population exhibits PPD response, and hence they possess host protective immune response. However, such immune response is not sufficient might be because inappropriate Tcm:Tem ratio. Thus, we would like test if a boost up dose of BCG along with clofazamin or nano-curcumin improves vaccine efficacy of BCG in preprimed population. This will be an enormous opportunity for improving vaccine efficacy simply by giving a boost up
Figure 1b. Bioavailability of nano curcumin. Mice were given I.P injections with curcumin/nanocurcumin (vehicle: PBS containing 10% ethanol) per mice at 100 mg/kg body weight and then bled at different time points via retro-orbital puncture and blood collected from each mouse and subjected to analysis by HPLC.
Figure 2. Lung and spleen CFU. Mice infected with a low-dose aerosol inoculum (~150 CFU/mice) of M.tb strain H37Rv and treatment of nano curcumin started 7 days post infection. Mice were sacrificed at various …show more content…
Inhibition of Tem gives an opportunity to generate more Tcm, which will enable better vaccine efficacy. Kv1.3 is preferentially expressed in effector memory T cells (Tem), and hence this can be targeted for the inhibition of Tem. A well-known inhibitor for Kv1.3 is clofazamine, which in used for several indications including TB. Therefore, we would like to test if addition of clofazamine during vaccination improves vaccine efficacy of BCG. Although clofazimine is an approved drug for several indications, it is not expectedly safe. It has a half life more than 28 days, and accumulates in different organs (19). Curcumin is also inhibitor for Kv1.3, however its bioavailability is poor and it failed in phase I clinical trials (20). Recently, we developed nano-curcumin which has longer bioavailability, and is more efficacious for TB therapy. Therefore, we would like to test if addition of nano-curcumin enhances BCG vaccine efficacy. Although, BCG is not an effective vaccine it’s being used since its inception. Therefore, a large number of people are already vaccinated. The vaccinated population exhibits PPD response, and hence they possess host protective immune response. However, such immune response is not sufficient might be because inappropriate Tcm:Tem ratio. Thus, we would like test if a boost up dose of BCG along with clofazamin or nano-curcumin improves vaccine efficacy of BCG in preprimed population. This will be an enormous opportunity for improving vaccine efficacy simply by giving a boost up