Myoblasts

I.A Background & Key Question(s) Rapid reproductions in muscles cells are needed because our muscles are constantly exposed to exercise and injury. In our muscles, Myoblast is the embryonic cells that mature and fuse together forming Myocytes. Through the process myogenesis, Myocytes form muscles. However, specifically satellite cells aid in new growth and regenerated muscle. Since these cells respond to one another and create a domino effect they were used in this experiment. Rozwadowska et al (2013) wanted to investigate the nuclear architecture of human primary myoblasts and myocytes in an in vitro culture, with reference to global changes in genomic expression. They were primarily looking to determine if the different muscle tissue cells would alter the architecture of the nucleus. Following that is to see if observed changes would change the movement of centromeres on the chromosomes.
I.B Techniques Used Many methods were used to complete this experiment. To begin, cell isolation occurred with human myoblast that was collected from a knee ligament surgery. Immunofluorescence was …show more content…
Centromeres on chromosomes 1, 3, 7, 11, 12, 17 and X were chosen to be examined because they are genes known to play a crucial role in the maintenance of myogenic characteristics of cells (Rozwadowska et al. 2013). They examined these organisms during different time periods; first during active reproduction of myoblasts and the second during the developmental stage of muscle fibers when it could no longer divide. Five of the seven centromeres had relocated while the other two did not. . Rozwadowska et al (2013) discovered that the centromeres on chromosomes 1,3 and 17 relocated to the nuclear perimeter, the centromeres on chromosomes12 and X relocated to intermediate shells of the nuclei and the centromeres on chromosomes 7 and 11 were the two that remained