Morphology Case Study

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Gross Morphology
At the time of sacrifice, all animals appeared healthy and showed no signs of infection, pain, or failed repairs. Two sheep from the ETG-RC repair group were excluded from the study. One of the sheep died, unexpectedly, of a C. perfringens type A enterotoxemia. The other sheep was sacrificed at an earlier time point to evaluate the progress of the repair. Gross evaluation of the infraspinatus tendon and humerus (Figure 4d-f) showed increased connective tissue in suture-only repaired shoulders compared to ETG-RC repaired shoulders. However, both repair techniques had more connective tissue compared to contralateral shoulders. The cross-sectional area of the infraspinatus tendon was measured and was significantly different
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However, radiographs showed evidence of heterotopic ossification, specifically, in the suture-only repair group, which was later confirmed by histology (Figure 6a). Opacity was semi-quantitatively analyzed, based on brightness, to evaluate calcification of the infraspinatus tendon. Contralateral shoulders of ETG-RC and suture only repaired shoulders were not significantly different and had a mean brightness gray scale value of 102.5 ± 5.6 and 106.8 ± 5.8, respectively. ETG-RC repaired shoulders were not significantly different from their respective contralateral shoulders and had a mean brightness gray scale value of 106.8 ±5.85. However, the suture only repairs had a value significantly higher than the respective contralateral shoulders (119.4 ± 6.4 gray scale units) (Figure …show more content…
H&E staining showed that the enthesis of ETG-RC repair was generally composed of graded zones that resembled native enthesis structure (Figure 4 G-L). This was not seen in the suture-only repairs, which were characterized by more disorganized scar-like tissue. Additionally, calcification was also present within the infraspinatus tendon…
Things Include today:
a) Histology of calcification in tendon (Alizarin)
b) Cellularity counts and representative image of fatty infiltration.

Biomechanical Testing
Following 6 months’ recovery, tears repaired with either ETG-RC or suture technique displayed no difference in stiffness between groups. All repair groups were significantly less stiff than contralateral shoulders (Figure 11). The mean stiffness of the tendon-bone interface was as follows: contralateral (, n=20), ETG-RC repair (, n=10), and suture-only repair (, n=11). Assessment of the biomechanical integrity of the tendon indicated that on average, the modulus of the ETG-RC repair was 11% higher compared to suture-only repair, but there was no statistically significant difference between moduli (p=0.33)

-Recapitualation of enthesis
-purpose of this study was to ..

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