Momordica Charantia Case Study
Objective of the Study:
General Objective To determine the anthelmintic activity of Momordica charantia aqueous leaf …show more content…
Scope and Limitation of the Study: This study would only focus on the aqueous leaf extract of the plant and not the plant itself as well as the detection of the active component that have rendered the anthelmintic activity.
500 grams of Momordica charantia leaves will be bought from a market place. It will be washed thoroughly with water followed by air drying. The leaves will be mashed afterwards. For the aqueous extraction, 500 g of the leaves will be placed in a blender to be homogenized together with 500 ml of distilled water. The processed leaves will be filtered twice using a filter paper and will be placed in a shaker for 24 hours. The filtrate will be then subjected to further extraction using rotary evaporator at a temperature of 97–98 degrees Celsius until the crude aqueous extract was left (Laborota 4001-Heidolph, serial no 069902956, type Heizbad WB no. 517.01000.00 0). It will be then diluted to several concentrations: 200µg/ml, 250 µg/ml, 500 µg/ml, 750 µg/ml, 1000 µg/ml, 1250 µg/ml, 1500 µg/ml, 1750 µg/ml, and 2000 µg/ml (Wongsawad et al., …show more content…
Simultaneously, some roundworms will be left unexposed on media M199 (negative control) to determine how long the roundworms would remain viable and alive in vitro. The roundworms will be exposed to the extract of Momordica charantia and will be statistically compared to the positive and negative controls (Duthaler et al., 2010) AQUEOUS LEAF EXTRACT CULTURE MEDIA (ml) EXTRACT (%) FINAL CONCENTRATION (ug/ml) 7.14 12.86 35.70 2000 6.25 13.75 31.25 1750 5.4 14.60 27.00 1500 4.5 15.50 22.50 1250 3.6 16.40 18.00 1000 2.8 17.20 14.00 750 1.8 18.20 9.00 500 0.9 19.10 4.50 250 0.7 19.03 3.50