Microbiology Lab Report Essay

1476 Words Mar 5th, 2013 6 Pages
Lab Report #1: Observing Bacteria Microbiology

Abstract:
This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x, 40x, and 100x (oil immersion) objective lenses, which allowed for the identification of the different organism’s shapes and sizes.
Purpose:
The aim of this exercise is to equip the student with the knowledge and skill necessary for conducting microscopic observations. It is also intended to teach the student the various sizes and shapes of several different types of bacteria.
Procedure:
1. Focus the microscope on a slide containing
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* Clips – hold the specimen in place. * Lamp – produces the light. * Condenser – a lens that focuses and aligns the light from the lamp to the specimen. * Diaphragm – is located in the light path to modify the measure of light reaching the condenser. * Objective lens – intakes the light from the specimen. * Eyepiece – to transmit and enlarge the image from the objective lens to your eye. * Nosepiece – a revolving support system that holds objective lenses. * Tube – holds the eyepiece at the appropriate distance from the objective lens and blocks out unwanted light. * Coarse-focus knob – brings the slide sample into the focal plane of the objective lens. * Fine-focus knob – makes the appropriate adjustments to focus the image. * Arm – a curved portion that holds all of the optical parts at a fixed distance and aligns them. * Base – is the bottommost part of the microscope that supports the weight of all the microscopic parts. * Tube – is connected to the arm and supports the eyepiece and objectives.

B. Definition of the following terms: * Focus: to position the objective lens at the proper distance from the specimen. * Resolution: The closest two objects can be before they are no longer detected as separate objects. * Contrast: How well the specimen stands out from the background.

C. Description of fresh yoghurt slide:

After leaving out

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