Microbial contamination is a constant problem, which often compromise development of all in vitro techniques. Therefor cleanliness and proper care of equipment are vital to the operation of a tissue culture laboratory to avoid the risk of contamination. In this practical, the main objective was to observe the sources of contamination and to learn maintenance techniques which are relevant to avoid the risk of contamination in the laboratory premises. While observing the cultures, cultures which were contaminated with bacterial growth and cultures which undergo browning were identified. As for the conclusion, this practical proved that maintaining cleanliness inside the laboratory is important to avoid contamination.
2.0 INTRODUCTION …show more content…
Microorganisms are ever-present. Bacteria can be isolated from nearly any surface including inanimate objects and human skin. Fungal spores and bits of vegetative hyphae drift into a laboratory from air conditioning ducts and open doors. Mycoplasma infections most frequently originate from improperly sterilized media or serum. At the risk of eliciting paranoia in the novice cell culture user who has no training in microbiological techniques, the possibility for microbial contamination exists everywhere. Inherent with successful manipulation of cell cultures is the basic understanding that everything that comes into contact with the cells must be sterile or no contaminating. This includes media, glassware, and instruments, as well as the environment to which the cultures are briefly exposed during transfer procedures. Because cleaning up a contaminated culture is too frequently a disheartening and unsuccessful experience, the best strategy is to employ procedures to prevent microbial contamination from occurring in the first place. (Coté, …show more content…
5.0 IMPORTANCE OF CARRYING OUT SUCH AN ACTIVITY
All the contaminations we found were due to bacteria. Bacteria and fungi, including molds and yeasts, are ever-present in the environment and are able to quickly colonize and flourish in the rich cell culture milieu. Their small size and fast growth rates make these microbes the most commonly encountered cell culture contaminants. In the absence of antibiotics, bacteria can usually be detected in a culture within a few days of contamination, either by microscopic observation or by their direct effects on the culture (pH shifts, turbidity, and cell death) (Leifert C. And Cassells A.C., 2000).
Bacterial contamination remains a continuing threat to plant tissue culture, but techniques for reducing contamination are available. Laboratories must assess their Situation, To determine contamination Sources, and change their laboratory operations to avoid or eliminate most of the contaminants (Leifert C. And Cassells A.C., 2000).
Properly training, operators in sterile technique and attending to the maintenance and use of autoclaves, laminar flow hoods and growth rooms are the first important steps toward avoiding environmental
2.0 INTRODUCTION …show more content…
Microorganisms are ever-present. Bacteria can be isolated from nearly any surface including inanimate objects and human skin. Fungal spores and bits of vegetative hyphae drift into a laboratory from air conditioning ducts and open doors. Mycoplasma infections most frequently originate from improperly sterilized media or serum. At the risk of eliciting paranoia in the novice cell culture user who has no training in microbiological techniques, the possibility for microbial contamination exists everywhere. Inherent with successful manipulation of cell cultures is the basic understanding that everything that comes into contact with the cells must be sterile or no contaminating. This includes media, glassware, and instruments, as well as the environment to which the cultures are briefly exposed during transfer procedures. Because cleaning up a contaminated culture is too frequently a disheartening and unsuccessful experience, the best strategy is to employ procedures to prevent microbial contamination from occurring in the first place. (Coté, …show more content…
5.0 IMPORTANCE OF CARRYING OUT SUCH AN ACTIVITY
All the contaminations we found were due to bacteria. Bacteria and fungi, including molds and yeasts, are ever-present in the environment and are able to quickly colonize and flourish in the rich cell culture milieu. Their small size and fast growth rates make these microbes the most commonly encountered cell culture contaminants. In the absence of antibiotics, bacteria can usually be detected in a culture within a few days of contamination, either by microscopic observation or by their direct effects on the culture (pH shifts, turbidity, and cell death) (Leifert C. And Cassells A.C., 2000).
Bacterial contamination remains a continuing threat to plant tissue culture, but techniques for reducing contamination are available. Laboratories must assess their Situation, To determine contamination Sources, and change their laboratory operations to avoid or eliminate most of the contaminants (Leifert C. And Cassells A.C., 2000).
Properly training, operators in sterile technique and attending to the maintenance and use of autoclaves, laminar flow hoods and growth rooms are the first important steps toward avoiding environmental