This lab makes use of the reaction excess powdered calcium carbonate and different concentrations limiting hydrochloric acid in order to determine the effect of changing concentration on the rate of the reaction. Students will carry out 3 trials of 5 experiments each trial. The five different experiments are for the various concentrations of hydrochloric acid (0.2, 0.4, 0.6, 0.8, 1.0 mol/L). First measure out approximately 2 grams of powdered calcium carbonate using a weighing boat and analytical balance. Then, measure out 30 mL of 0.2 M hydrochloric acid into a volumetric flask.…
Sterols in plants are almost completely derived from acetyl-coenzyme A (acetyl-CoA). Acetyl-CoA is dimerized to acetoacetyl-CoA that then forms 3- hydroxy-3-methylglutaryl-CoA (HMG-CoA) by a condensation reaction. HMG-CoA is reduced to mevalonic acid by 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), a rate-limiting step in human isoprenoid biosynthesis. HMGR over-expression in transgenic tobacco plants resulted in increased levels of total sterols, indicating that HMGR is a limiting enzyme also in plant sterol biosynthesis (Schaller et al., 1995). The next step in sterol synthesis is to form squalene from mevalonic acid, and this is performend by a series of reactions where the final step is catalysed by the squalene synthase enzyme (SQS).…
The goal of experiment four was to use sodium dichromate to oxidize borneol to camphor. The camphor was purified using sublimation, then reduced to isomeric alcohol isoborneol with sodium borohydride. 1H NMR was used to determine the ratio of borneol to isoborneol in the final product. The experiment was carried out by using sodium dichromate to oxidize a borneol solution. Once the reaction was complete the mixture was separated using extraction techniques, dried, and ether was removed via the rotovap.…
solution, because it is the source of the Grignard reagent. Because the halide cannot be eliminated, the next best option is to minimize the amount of halide in the reaction. In this experiment, a small concentration of the halide is reacted with a high concentration of magnesium to ensure maximum contact between halides and magnesium. To do this, magnesium will be placed in the round bottom flask and then halides are slowly added. Another potential source of error is that Grignard reagents must be placed far away from water sources.…
Doc. Anthony Miller, a physicist, and an engineer, with him Doc. Stephen Strange who had the same expertise as Doc. Anthony Miller. They were transported to Area 51 which was a secret military facility, they were brought there to work on a project.…
This is most near the retention time of n-Butyl benzoate, which is 3.38 minutes. As such, it is likely that the unknown ester is n-Butyl benzoate. This would mean that the acid was benzoic…
R.C. 5.9 Mathematics (Mobile Infant) Activity: “Stack Them Up” Materials: Stacking blocks, nesting cups, measuring cups Infants are continually growing in all areas of development. In this activity, the goal is to get the infant to stack their blocks or cups. The teacher would have the child sit on the floor or at a table with their toys.…
Abstract: The Grignard reaction was successfully accomplished. An amount of 1.20 ml of 2-methyl-2-pentanol was collected, and 9.60% yield was calculated based on the theoretical yield. Despite the low amount of 2-methyl-2-pentanol, its identity was confirmed by comparing the experimental refractive index and infrared spectrum to the literature refractive index and infrared spectrum of 2-methyl-2-pentanol. Statement and purpose The purpose of this experiment is to synthesize a specific alcohol by using the Grignard reaction.…
Purpose The purpose of this lab was to separate unknown compound 204, composed of an amine, a carboxylic acid, and a neutral component, into each of its three respective states. The unknown compound was separated using microscale separation of acidic, basic, and neutral components by liquid/liquid extraction. Liquid/liquid extraction is a very common method often used in work-ups and allows for isolation of neutral components using acid/base chemistry; this method is extremely helpful in separating reactants and products of a synthesis. The components were purified and dried by evaporation and vacuum filtration.…
After starting with 30 mL of an unknown mixture of two solvents, fractional distillation and gas chromatography were used to identify the unknowns as acetone and toluene in a 1 to 2.04 ratio of acetone to toluene. Fractional distillation was first used to separate the unknown mixture into its components based on boiling point. Distillation refers to the method of separating components of a mixture through heating and cooling. Simple distillation, which is especially useful in the separation of less-volatile substances from volatile substances, such as sugar from water, can be used to separate a liquid-liquid mixture if the boiling points of the component liquids differ by more than 40-50°C. A plot of temperature versus volume for a simple…
Subject: Experiment 23: Stereochemistry of the Addition of Bromine to trans-Cinnamic Acid Introduction/Abstract: The purpose of this experiment was to carry out the bromination of trans-cinnamic acid, to determine the stereochemistry of the dibromide product of 2,3-dibromo-3-phenylpropanoic acid, and find out whether the reaction proceeds by the usual bromonium ion mechanism or some other mechanism. In this experiment trans-cinnamic acid was mixed with glacial acetic acid and stirred in which then bromine/acetic acid solution was added to the mixture little by little. The dibromide becomes the precipitate of this solution during the reaction. Once this precipitate is separated by vacuum filtration the melting point helps identify…
Introduction The purpose of this experiment was to understand acid-catalyzed hydration of alkenes through the formation of exo-norborneol from the reaction of Norborene with acid and the excess of water. Acid-catalyzed hydration is useful in removing double and triple bonds; an unsaturated compound becomes reduced to a saturated compound in the presence of the acid catalyst, or water for this experiment. Hydration of alkenes is the result of acid-catalyzed addition of water to a carbon-carbon double bond. This leads to the formation of an alcohol.…
By combining the unknown compound with an acid, a base, and two salts, its reactivity was determined. Reacting the unknown compound with sulfuric acid (H2SO4) produced no visible reaction (precipitate), although the pH of the resulting solution was very acidic. Combining the unknown compound with sodium hydroxide (NaOH), a strong base, also had no visible reaction. However, the resulting solution was a lot more basic. Reacting the unknown compound with sodium nitrate (NaNO3), a salt, did not have a visible reaction nor did the pH of the resulting solution change.…
Abstract: The objective of this experiment was to synthesize aspirin from salicylic acid and acetic anhydride. The general theory behind this experiment was to study the synthesis of a drug from organic materials. During the experiment, esterification had occurred between reactants salicylic acid and acetic anhydride. Then, phosphoric acid would catalyze the reaction and water would be added to decompose the remaining acetic anhydride. Through the process of filtration, aspirin crystals were collected and separate from the liquid acetic acid-water solution.…
The lab we conducted involved a process called the Maillard Reaction. When it comes to cooking meats, heating the food causes the proteins, especially those nearest the surface of the meat, to denature. These denatured proteins become more chemically reactive to other molecules in their environment. One of the primary reactions that occur when browning meats and many other foods is called the Maillard Reaction.…