Essay on Metabolism and Oxidative Cellular Respiration

1813 Words Dec 13th, 2013 8 Pages
Metabolism III: Oxidative Cellular Respiration

Introduction

Oxidative cellular respiration is composed of series metabolic processes that convert broken down food molecules into usable energy in the form of adenosine tri-phosphate (ATP). The process follows oxidation (catabolic) and reduction (anabolic) pathways. Processes involved are glycolysis, Krebs or tricarboxylic acid (TCA) cycle, and the electron transport chain. One step in the TCA cycle is the enzyme-catalyzed conversion of succinate to fumarate in a redox reaction. In intact cells succinate loses hydrogen ions and electrons to FAD to form fumarate. This step in the TCA cycle will be used to study the rate of cellular respiration under different conditions.
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No succinate was added to Tube1 (blank).

Results

*The spectrophotometer is set for optimum transmission level for (DPIP) therefore 100% transmission refers to (DPIP) in its fully oxidized state.

*The spectrophotometer is set for optimum transmission level for (DPIP) therefore 100% transmission refers to (DPIP) in its fully oxidized state.

When looking at the results in Table 1, and Figure 1 we can compare the percent transmission of the three solutions in test tubes 1, 2, 3 respectively over a time period of 30 minutes. The increase in percent transmission is a result of increased cellular respiration activity indicated by the reduction of dichlorophenol-indophenol (DPIP), resulting in a colorless effect that is measured by the amount of light passing through or percent transmission recorded. Tube 3 has the highest reaction rate of 2.10%/min representing the highest rate of cellular respiration comparatively. This trend is continued throughout the results, test tube 1 has 0ml succinate yielding the lowest rate of cellular respiration, tube 2 has 2.0ml succinate yielding the second highest cellular respiration rate, and tube 3 has 3.0 ml succinate yielding the highest cellular respiration rate.

Figure 2: Effect of pH 2, 4, 6, 8, and 10 on cellular respiration rate The results provide information about optimal environmental pH levels for cellular respiration to occur. Respiration occurred in pH 2, 6, 8, and

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