Merck Research Paper

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9.3. Lauryl tryptose broth (MERCK)
Tryptose ..................................................................................................................... 20.0 g
Lactose.........................................................................................................................5.0 g
Dipotassium hydrogen phosphate, K2HPO4 ..............................................................2.75 g
Potassium dihydrogen phosphate, KH2PO4...............................................................2.75 g
Sodium chloride, NaCl................................................................................................5.0 g
Sodium lauryl sulfate ..................................................................................................0.1 …show more content…
9.5. MFC medium (MERCK)
Tryptose or biosate …………………………………………......................................10.0 g
Proteose peptone No. 3 or polypeptone …………………………………………….. 5.0 g
Yeast extract ………………………………………………………………………... 3.0 g
Sodium chloride, NaCl ……………………………………………………………… 5.0 g
Lactose ………………………………………………………………………………12.5 g
Bile salts No. 3 or bile salts mixture …………………………………………………1.5 g
Aniline blue …………………………………………………..................................... 0.1 g
Agar (optional) ………………………………………………………………….. 15.0 g
Distilled water …………………………………………………..………………….1 L pH should be 7.4±0.2 after sterilization by heating.
9.6. EC medium (MERCK)
Tryptose or trypticase ..............................................................................................20.0 g
Lactose.................................................................................................................... 5.0 g
Bile salts mixture or bile salts No. 3 ...................................................................... 1.5 g
Dipotassium hydrogen phosphate, K2HPO4 ......................................................... 4.0 …show more content…
Brain– heart infusion agar (BHIA) (LAB M)
Brain Heart Infusion (Solids) ............................................................................ 8 g
Enzymatic Digest of Animal Tissue .................................................................. 5 g
Enzymatic Digest of Casein .............................................................................. 16 g
Dextrose ............................................................................................................. 2 g
Sodium Chloride ................................................................................................ 5 g
Disodium Phosphate .......................................................................................... 2.5 g
Agar ................................................................................................................... 13.5 g pH should be7.4±0.2 afterautoclaving at 121°C for 15 min.
9.11. Tryptic Soy Agar (LAB M)
Tryptone (Pancreatic Digest of Casein)...........................................................15.0 g
Soytone (Papaic Digest of Soybean Meal)…………………………………..5.0 g
Sodium Chloride…………………………………………………………….5.0 g
Agar………………………………………………………………………….15.0 g pH should be 7.3 ± 0.2 after autoclaving at 121°C for 15

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