3. Measurement of glycated albumin
As mentioned before, the measured albumin is considered a reliable tool to supplement other methods of measuring blood glucose, such as HbA1c and standard assays for glycemic control and diagnosis diabetes [30]. The HbA1c lifespan is estimated to be about 90 to 120 days. Thus, it is known as an indicator for the assessment of long-term glycemic control [17, 31]. However, the level of HbA1c is not suitable for the assessment of the patient’s status in some situations [32]. All of these limitations in current approaches forced researchers to discover another indicator to evaluate glycemic control, especially for examining blood sugar control over a shorter period of time [17]. GA was proposed as a new marker with shorter life expectancy (12 to 21 days) [17, 30, 31]. This means that GA levels can monitor the patient 's condition for 2 to 3 weeks [4].
There are many methods for measuring GA [33], including immunoassay-related techniques (such as enzyme linked immunosorbent assays) [17, 34], boronate affinity chromatography[17, 35], high-performance liquid chromatography (HPLC) …show more content…
In all cases, when hemin was connected to the albumin-functionalized surface, QCM-D data showed low frequency and high dissipation. The report showed that when the hemin was exposed to an albumin-functionalized surface, noticeable irreversible changes in frequency occurred instantaneously. They believed the first exposure of hemin capped unintended irreversible binding sites. Therefore, the change in frequency reported by QCM-D measurements taken place during the second exposure of hemin was large, and it is caused by binding to reversible sites. No difference was observed between the nonglycated control and HSA glycated with glucose, which primarily attached to the lysine
As mentioned before, the measured albumin is considered a reliable tool to supplement other methods of measuring blood glucose, such as HbA1c and standard assays for glycemic control and diagnosis diabetes [30]. The HbA1c lifespan is estimated to be about 90 to 120 days. Thus, it is known as an indicator for the assessment of long-term glycemic control [17, 31]. However, the level of HbA1c is not suitable for the assessment of the patient’s status in some situations [32]. All of these limitations in current approaches forced researchers to discover another indicator to evaluate glycemic control, especially for examining blood sugar control over a shorter period of time [17]. GA was proposed as a new marker with shorter life expectancy (12 to 21 days) [17, 30, 31]. This means that GA levels can monitor the patient 's condition for 2 to 3 weeks [4].
There are many methods for measuring GA [33], including immunoassay-related techniques (such as enzyme linked immunosorbent assays) [17, 34], boronate affinity chromatography[17, 35], high-performance liquid chromatography (HPLC) …show more content…
In all cases, when hemin was connected to the albumin-functionalized surface, QCM-D data showed low frequency and high dissipation. The report showed that when the hemin was exposed to an albumin-functionalized surface, noticeable irreversible changes in frequency occurred instantaneously. They believed the first exposure of hemin capped unintended irreversible binding sites. Therefore, the change in frequency reported by QCM-D measurements taken place during the second exposure of hemin was large, and it is caused by binding to reversible sites. No difference was observed between the nonglycated control and HSA glycated with glucose, which primarily attached to the lysine