We have carefully evaluated anti-inflammatory and anti-arthritic activity of Mangiferin (NF-κB inhibitor) and its interactions with low dose of NO modulators (L-NAME and L-Arginine) for the treatment of adjuvant-induced experimental arthritis in rats.
Maximum anti-inflammatory and anti-arthritic activity of Mangiferin was observed in rats treated with Mangiferin 30 mg/kg. Its protective effect was potentiated by combination with low dose of NO modulator L-NAME (10 mg/kg). Treatment with Mangiferin has improved general quality of life of CFA inoculated rats and has prevented and restored the loss in body weight.
Increase in ROS/RNOS activity in CFA inoculated rats, results in significant increase in MDA levels; while SOD activity is markedly suppressed. Mangiferin due to its ROS/RNOS inhibiting property decreases MDA levels, while SOD activity is restored significantly. Mangiferin regulates oxidative stress by (1) modulating Nrf2/antioxidant response element (ARE) detoxification pathway; (2) directly detoxifying reactive species such as ROS and RNS; (3) activating detoxification enzymes such as catalase. In addition, Mangiferin inhibits …show more content…
Findings, of this study are in agreement with earlier studies which have reported role of NO in inflammatory/auto-immune disorders. Our study, provides the evidence that inhibition of NO production is one of the factor involved in the anti-inflammatory and anti- arthritic effects of Mangiferin. Anti-arthritic and anti- inflammatory effect of Mangiferin was further potentiated by its combination with low dose of NO modulator L-NAME. L-NAME when given in low dose exerts its anti- inflammatory effect by inhibiting NO-NOS pathway which promotes inflammation by activating various inflammatory mediators such as cyclooxygenase (Jarazo Dietrich et al.