Introduction
In the experiment directed by Jeffrey Peters et al, two distinct species of ducks were studied. One group, the mallard ducks, has a great population size with sightings in nearly all North America, Asia, and Europe. The other group, the mottled ducks, are found in much smaller numbers and can only be seen in Florida and the Western Gulf Coast (WGC). These species are known to hybridize and share a recent ancestor. In order to study their genetic diversity six distinct loci were analyzed, taking into account their population size and gene flow. This was specifically done with the North American mallards and the WGC mottled ducks.
Methods
Sampling and DNA Sequencing
99 mallard ducks were sampled in the fall within six different areas of North America, while 190 mottled ducks were sampled in eight locations throughout Texas and Louisiana. The scientist utilized published information in addition to analyzing the same factors in their study. This included mtDNA control region sequences. PCR was also used to determine the sex of the ducks. The new sequences discovered have been submitted to GenBank. The software titled Sequencher v.5.0.1 and PHASE v.2.1.1 were also used to analyze the sequences. …show more content…
They included: ARLEQUIN v.3.5.1.2 (to examine variety in nucleotide and genes), HP-RARE v.1.0 (to regulate the uneven selection size), Microsoft Excel (used in comparison of each measure of genetic diversity), AMOVA (three levels, each used to measure population disparity), STRUCTURE v.2.2.4 (to measure loss of Hardy-Weinberg equilibrium) and STRUCTURE HARVESTER (to determine the delta number of genetic
In the experiment directed by Jeffrey Peters et al, two distinct species of ducks were studied. One group, the mallard ducks, has a great population size with sightings in nearly all North America, Asia, and Europe. The other group, the mottled ducks, are found in much smaller numbers and can only be seen in Florida and the Western Gulf Coast (WGC). These species are known to hybridize and share a recent ancestor. In order to study their genetic diversity six distinct loci were analyzed, taking into account their population size and gene flow. This was specifically done with the North American mallards and the WGC mottled ducks.
Methods
Sampling and DNA Sequencing
99 mallard ducks were sampled in the fall within six different areas of North America, while 190 mottled ducks were sampled in eight locations throughout Texas and Louisiana. The scientist utilized published information in addition to analyzing the same factors in their study. This included mtDNA control region sequences. PCR was also used to determine the sex of the ducks. The new sequences discovered have been submitted to GenBank. The software titled Sequencher v.5.0.1 and PHASE v.2.1.1 were also used to analyze the sequences. …show more content…
They included: ARLEQUIN v.3.5.1.2 (to examine variety in nucleotide and genes), HP-RARE v.1.0 (to regulate the uneven selection size), Microsoft Excel (used in comparison of each measure of genetic diversity), AMOVA (three levels, each used to measure population disparity), STRUCTURE v.2.2.4 (to measure loss of Hardy-Weinberg equilibrium) and STRUCTURE HARVESTER (to determine the delta number of genetic