Abstract This experiment was constructed to distinguish the differences with Prokaryotes and Eukaryotes by growing through a variety of media. These media consisted of rich and minimal. Rich media is a media that contains an abundant supply of nutrients for bacteria to grow. Minimal media is a media that contains the minimal amount of nutrients for bacteria to grow. Medias are important because they not only are used in growing microorganisms but are used in the medical field to help with the treatment of viruses. For Prokaryotes growth we oversaw the growth of Vibrio natriegens for 2 hours and collected samples every 10 minutes in order to determine growth through turbidity and viable cell counts. For Eukaryotes growth, we looked at Saccharomyces
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By conducting this experiment, we concluded that the longer we left the bacterium Vibrio natriegens colonizing, the more CFU/mL we observed. The plots represent time points during our experiment in which we recorded the CFU/mL for that allotted time. The intervals in which we recorded information were set for every 10 minutes.
Figure 1b
Eukaryotic Growth.
Through this experiment we were able to conclude that as the longer S. cerevisiae was let to colonize in the minimal media, the more cells/mL were found. This is clearly shown in the table demonstrating the number of plate counts that were found. Each plot on the table represents an allotted hour that we took a recorded measurement of the cell growth. Time points were designated for every 4 hours.
1.2 Prokaryotic Optical Density
Figure 2:
Prokaryotic Optical Density (600nm).
For the prokaryotic growth, optical density increased in a linear fashion as the time increased. The plots represent time intervals of 10 minutes recorded throughout the experiment to help determine the optical density of Vibrio natriegens over a time of 120 minutes.
1.3 Eukaryotic Growth between Rich and Minimal Media
Figure
By conducting this experiment, we concluded that the longer we left the bacterium Vibrio natriegens colonizing, the more CFU/mL we observed. The plots represent time points during our experiment in which we recorded the CFU/mL for that allotted time. The intervals in which we recorded information were set for every 10 minutes.
Figure 1b
Eukaryotic Growth.
Through this experiment we were able to conclude that as the longer S. cerevisiae was let to colonize in the minimal media, the more cells/mL were found. This is clearly shown in the table demonstrating the number of plate counts that were found. Each plot on the table represents an allotted hour that we took a recorded measurement of the cell growth. Time points were designated for every 4 hours.
1.2 Prokaryotic Optical Density
Figure 2:
Prokaryotic Optical Density (600nm).
For the prokaryotic growth, optical density increased in a linear fashion as the time increased. The plots represent time intervals of 10 minutes recorded throughout the experiment to help determine the optical density of Vibrio natriegens over a time of 120 minutes.
1.3 Eukaryotic Growth between Rich and Minimal Media
Figure