In our study, we had two controls that we placed the Lumbriculus variegatus into. First, we used spring water to get the normal heart rate of the worms. The other control was a caffeine solution that served as our positive control. Our other solutions that we exposed the worms to consisted of increasing concentrations of two different NSAIDs (ibuprofen and aspirin). For each NSAID, we had a solution equivalent to the recommended dosage for a person based on body weight (the proportional dosage solution), ten times the concentration of this initial solution based on the body weight of the worm, and one-hundred times the concentration of the solution based on the average weight of Lumbriculus variegatus. The Lumbriculus variegatus were immersed in each solution for a set time of ten minutes. Then, the worms
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We then calculated the heart rate of each Lumbriculus variegatus.
Preparation of Solutions:
For our study, we prepared the solutions to be proportional to the dosage a Lumbriculus variegatus would receive based on its weight. For all of our trials, we used the value of 8 mg for the weight of one Lumbriculus variegatus, because that is their average weight. We made a 2.5 millimolar solution of caffeine by diluting 1 mL of caffeine in 30 mL of spring water. For our spring water control, we measured out 30 mL of spring water for each trial (two trials total). For our NSAID solutions of ibuprofen and aspirin, we calculated the dosage a Lumbriculus variegatus would receive. A single tablet of aspirin is 325 mg per human adult and an adult weighs on average 62 kg, so the dosage is 0.05 mg of aspirin/g of body weight. We made a stock solution to prepare our aspirin solutions of various concentrations. To do this, we dissolved
We then calculated the heart rate of each Lumbriculus variegatus.
Preparation of Solutions:
For our study, we prepared the solutions to be proportional to the dosage a Lumbriculus variegatus would receive based on its weight. For all of our trials, we used the value of 8 mg for the weight of one Lumbriculus variegatus, because that is their average weight. We made a 2.5 millimolar solution of caffeine by diluting 1 mL of caffeine in 30 mL of spring water. For our spring water control, we measured out 30 mL of spring water for each trial (two trials total). For our NSAID solutions of ibuprofen and aspirin, we calculated the dosage a Lumbriculus variegatus would receive. A single tablet of aspirin is 325 mg per human adult and an adult weighs on average 62 kg, so the dosage is 0.05 mg of aspirin/g of body weight. We made a stock solution to prepare our aspirin solutions of various concentrations. To do this, we dissolved