Lactobacillus And Bifidobacterium: Case Study

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Lactobacillus is the most commonly used bacterial species (Swyers et al. 2008). A study conducted by Al Jassim et al. recorded that several species of Lactobacillus were located throughout the equine gastrointestinal tract. This concludes that these microbes have the ability to adapt to pH changes and survive in different environments. L. mucosae and L. salivarius were found to be the most abundant. L. salivarius, specifically, was cultured from the stomach, colon, and rectum, suggesting its high survival rate in acidic environments (Al Jassim et al. 2005). While probiotics are supposed to be the “good” bacteria, there are certain strains of Lactobacillus and Bifidobacterium that are considered to be detrimental to the horse 's health. A study …show more content…
Without one, we would not be able to correctly identify or carry out our next step. The goal for Stage One is to ensure that the products that we are utilizing to dose our batch culture and our horses will be accurately represented on the labels. This step maximizes our success of identifying and using the most beneficial supplement, and aids us in our preparation for Stage Two. Our goal for Stage Two is to examine the performance of three widely used probiotic products when dosed into an in vitro model at recommended levels. It is important that discover which of the products tested will significantly alter the bacterial profile. By using modern pyrosequencing technology, we will be able to view the microbes as they are dosed in cecal fluid. This step could potentially uncover previously unknown details about their reactions and performances in this particular environment. This in vitro model will be a novel first step into examining the performance of these products on equine cecal fluid in batch culture as well as aid in discovering which microbes will be best suited in the gastrointestinal tract. Our goal for Stage Three is to provide horse owners and veterinarians with validated information that will help to guide recommendations and dosage levels for the probiotic tested. Many product labels are either very vague, confusing, or mislabeled. This can lead to inconsistent and poor use of these types of products, and can also be very misleading. By verifying the point at which the maximum benefit is yielded to the equine microbiome, we can help to educate the entire equine industry around the world. This data will impact for the equine and veterinary communities

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