Essay on Lab Report

1405 Words Nov 3rd, 2014 6 Pages
Roy Levin
Bio 11 Lab
Dr.Izquierdo
Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods
The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4, 0.8, 1.2, 1.6, 2.0 mg/ml of bovine serum were used to determine absorbance with a spectrophotometer. Two additional samples were made; one was blank and the other was for the specific homogenate sample. Then 3 microliters of bradford assay reagent, which indicates the amount of protein present
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The absobances of of the enzyme-treated and free-glucose samples were measured. By using the standard curve of the glucose linear equation, the concentration of free-glucose and total glucose was found. In order to find glycogen concentrations, total glucose was subtracted from free glucose. This concentration was multiplied by two to account for the dilution. The liver had the most glycogen conentration (2.499 mg/ml) comapred to the heart(0.684 mg/ml) and kidney(0.904 mg/ml)(Figure 4)
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Figure 4. Concentration of glycogen is shown in the heart, kidney, and liver homogenates of bos taurus. Errors bars were created from 5 readings for heart, 6 readings for liver, and 7 readings for kidney.
Free glucose were more abundant in the liver with a concentration of 1.451 mg/ml compared to the heart (.3129 mg/ml) and kidney (.2287mg/ml). Free glucose concentrations were determined by using the non-enzyme treated sample. Using the standard curve equation, concentration was found and multiplied by 2 to account for dilution.

Figure 5. Free glucose concentrations of heart, kidney, and liver homogenates of bos taurus. Errors bars were created from 5 readings for heart, 6 readings for liver, and 7 readings for kidney.
Figure 4 and 5 both indicated a similar pattern. The liver for glycogen had a concentration of 2.499 mg/ml and a free glucose concentration of 1.451 mg/ml. When compared to the heart and liver, the heart

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