The purpose of this lab is to separate two unknown bacteria and identify each from an unknown bacterial mixed culture and to determine which tests to do when identifying bacteria. Specific tests will lead to a definite answer as to what two bacteria was being dealt with. Unknown bacterium A was selected and subjected to isolation techniques and differential tests for identifying markers. Each experiment served to help eliminated other possible known bacteria and match each of the unknown bacteria two their known bacterium. A Gram stain was performed in which the unknown bacteria were found to be a Gram positive long rod and a Gram negative short rod. Using SIMs test, endospore stain and a nitrate test, the unknown Gram positive long rod bacterium
