Lab Report : Staphylococcus Aureus Essay

757 Words Apr 29th, 2016 4 Pages
Table 1 shows the all the isolates that produced a zone of inhibition for the ESKAPE pathogen, Staphylococcus aureus. In total, twenty six isolates were tested against Staphylococcus aureus. However, only eight showed a zone of inhibition. The size of the zones varied for each microorganism.

Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were non-endospores.
DISCUSSION
The purpose of this experiment was to search through soil, isolate and characterize new antibiotic producers. A soil sample was taken about 6 cm in depth from a nonagricultural land. Serial dilutions ranging from 10-6 to 10-4 were made using the soil. Thirty one colonies grew on the 10-4 plate and sixteen colonies grew on the 10-5 plate. No colonies grew on the 10-6 plate. The CFU/ gram of soil that was calculated was 3.1 x 10 6. The colonies varied in shapes, sizes and colors. Between the 10-4 plate and the 10-5 plate, twenty six colonies were chosen. These colonies were used for patch plating. On LB agar and 250 mg/mL cycloheximide plates, about twelve colonies were individually placed on the plates. The cells on this plate became…

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