Table 1 shows the all the isolates that produced a zone of inhibition for the ESKAPE pathogen, Staphylococcus aureus. In total, twenty six isolates were tested against Staphylococcus aureus. However, only eight showed a zone of inhibition. The size of the zones varied for each microorganism.
Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were non-endospores.
DISCUSSION
The purpose of this experiment was to search through soil, isolate and characterize new antibiotic producers. A soil sample was taken about 6 cm in depth from a nonagricultural land. …show more content…
This isolate did not reduce sulfur, no indole was formed and there was no motility. A FTM test was performed and this tested the aerotolerance of the microorganism. The results of this test was that the microorganism grew more towards the top of the tube. This means that the microorganisms is an aerobe. An oxidase test was performed on the isolate in order to determine if the microorganism can produce the enzyme cytochrome. The results were that the microorganism did not produce the enzyme cytochrome. A catalase test was performed on the microorganism and the microorganism produced bubbles. This means that this microorganism produces the catalase enzyme. In order to determine if the microorganism can tolerate high salt environments, it was streaked on an MSA plate. The microorganism grew on the plate indicating that it can grow in high salt concentrations. The blood agar plate was used to test the microorganism for hemolytic
Table 2 shows the results of the biochemical and morphological tests. The test that were performed were Sim, FTM, oxidase, catalase, MSA, blood agar, Acid fast stain and endospore stain. The sim and oxidase test were negative. The FTM, catalase, MSA and blood agar tests were positive. After the Acid Fast stain, the cells were blue and non-acid fast. After the Endospore stain, the cells were pink. This indicated that they were non-endospores.
DISCUSSION
The purpose of this experiment was to search through soil, isolate and characterize new antibiotic producers. A soil sample was taken about 6 cm in depth from a nonagricultural land. …show more content…
This isolate did not reduce sulfur, no indole was formed and there was no motility. A FTM test was performed and this tested the aerotolerance of the microorganism. The results of this test was that the microorganism grew more towards the top of the tube. This means that the microorganisms is an aerobe. An oxidase test was performed on the isolate in order to determine if the microorganism can produce the enzyme cytochrome. The results were that the microorganism did not produce the enzyme cytochrome. A catalase test was performed on the microorganism and the microorganism produced bubbles. This means that this microorganism produces the catalase enzyme. In order to determine if the microorganism can tolerate high salt environments, it was streaked on an MSA plate. The microorganism grew on the plate indicating that it can grow in high salt concentrations. The blood agar plate was used to test the microorganism for hemolytic