Db Enzyme Lab Report

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Sarah Gourgues
BIO LAB 1130.036
December 1, 2016
They call me DJ Enzyme because I 'm always breaking it down
Abstract:
Although protein function is thought to depend on the dynamics of the molecule and its environment contribute to catalytic mechanisms. The increase and decrease of temperatures affected the rate of reaction. In nature, organisms adjust the conditions of their enzymes to produce an optimum rate of reaction, where necessary, may have enzymes which are adapted to function well in extreme conditions where they live, to a certain maximum temperature. Temperature is a measure of kinetic energy of the molecule in a system. As the temperature increases, the kinetic energy and thus the number of random collisions of enzyme with …show more content…
The activity changes as measures for the absorbance and temperatures shown in Figure 1 below. Each cuvette contains the same solution, but all were tested at different temperatures measured in Celsius. Cuvette 1 is labeled as 0 Degrees Celsius which is shown below in Figure 1. The overall change in absorbance was higher than the room temperature controlled solution. Cuvette 2 is labeled as 23 Degrees Celsius and is shown below in Figure 1. This substance was used as the control throughout the experiment to compare the increasing or decreasing temperatures to receive the most accurate results. Cuvette 3 is labeled as 30 Degrees Celsius and is also shown below in Figure 1. The results from this temperature show a higher change in absorbance, but not the overall largest change compared to the controlled solution. Cuvette 4 is labeled as 72 Degrees Celsius and is shown below in Figure 1. The results from this temperature showed an increasingly low change in absorbance compared to the other temperatures tested. Knowing this information gives us a better understanding of why enzymes denature over time and can give scientists an idea of the ideal temperature to find the highest change in absorbance without allowing an enzyme to become unable to perform at its highest potential. More evidence to support this conclusion can be shown in Figure 2. This graph is measured during the middle of the timed …show more content…
"The Influence of Time, Storage Temperature, and Substrate Age on Potential Soil Enzyme Activity in Acidic Forest Soils Using MUB-linked Substrates and L-DOPA." June 2009. Web. 09 Nov. 2016.
McKinney, Susan. "Introduction to Enzymes." Temperature Effects. Worthington Biochemical Corporation, n.d. Web. 05 Nov. 2016.
Parker, Robert. "Enzymes." Chemistry for Biologists:. Royal Society of Chemistry, n.d. Web. 09 Nov. 2016.
Santhosh, Lakshmi. "The Effects of Temperature on Enzyme Activity and Biology | The Classroom | Synonym." The Effects of Temperature on Enzyme Activity and Biology | The Classroom | Synonym. Synonym, n.d. Web. 09 Nov. 2016.
Studios, Andrew Rader. "Enzymes Make the World Go 'Round." Chem4Kids.com: Biochemistry: Enzymes., n.d. Web. 09 Nov. 2016.
Williams, Lippicott. "A Systematic Evaluation of the Effect of Temperature: Journal of Trauma and Acute Care Surgery." LWW., 2004. Web. 05 Nov.

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