Lab Report On Green Photosynthesis Of Gold Nanoparticles Using Aqueous Extract Of Baker 's Yeast

1642 Words Aug 31st, 2016 7 Pages
3.1. Green photosynthesis of gold nanoparticles using aqueous extract of Baker’s yeast:
One aim of the present study is the development of an improved method for derivatization of metabolites existed in aqueous extract of Baker’s yeast (Saccharomyces cerevisiae). This procedure facilitates identification of metabolites in Baker’s yeast extract and its utility for the synthesis of nanomaterials. The preparation of trimethylsilylenol ethers of metabolites was performed by treatment of the extract with catalytic amounts of trimethylsilylchloride (TMSCl) and trimethylsilylimidazole to produce silylated compounds that were determined using gas chromatography mass spectroscopy. The GC/MS analysis of the culture supernatants of aqueous extract of Saccharomyces cerevisiae shows analysis for metabolites mass library (Figure 2).
When the gold ions (e.g. AuCl3) were added to the aqueous Baker’s yeast extract and then allowed to react under visible light for 30 minutes, it was observed that Au ions were reduced extracellularly to gold nanoparticles (figure 4). Au nanoparticles with uniform size distribution 13.0±0.9nm and plasmon band λ=~535nm are formed.
The crystalline nature of biosynthesized gold/yeast nanoparticles was further confirmed from XRD analysis. The XRD patterns of the photo-biosynthesized gold nanoparticles are shown in figure 5. The four intense diffraction peaks were observed at 2Ө values of 38.30°, 44.43°, 64.67°, and 77.67°, corresponding to the (111), (200), (220),…

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