Introduction
Antibiotics are the natural substances produced by the microbes that destroy or slow down the growth of the bacteria, whereas antimicrobial agents are the synthetic substances which developed in the laboratory that mimic effects of antibiotics. Selective toxicity means the microbial agents that kill the microbes without killing the host cells. Antimicrobial is divided into two spectrum of antibiotics –
1 Narrow spectrum: - This antibiotic is active against a selected group of the bacteria and will cause less resistance (SRS. n.d)
2 Broad spectrums: - This antibiotic is effective against a wide range of infectious micro-organisms which includes both gram positive and negative bacteria. (SRS. n.d)
The relative …show more content…
-Antibiotic disk of gentamycin, novobiacin and penicillin
-Nutrient agar in petri plate.
-Metric ruler, markers and plastic tweezers.
-Tea candle light, sterile swab, rubbing alcohol and bleach
-Other includes personal protective equipment like an apron, gloves, and safety goggles.
Kirby Bauer diffusion is widely used test for antibiotic effectiveness in certain kind of organisms. The steps I followed to complete this test are-
- First collect all the materials then wash the hands thoroughly and put the personal protective equipment.
- Clean the working surface to prevent from cross contamination.
- Take out the petri plate with nutrient agar in it. Divide the petri plate into three equal sections with permanent marker and label the antibiotic name (gentamicin, novobiacin and penicillin) in each section.
-Remove the lid of the active S epidermis broth, flame the lip of the tube with help of the tea light candle and careful remove the sterile swab and submerge the swab into the active S epidermis broth. -Swab the entire agar plate including the edges and let the active broth absorbed in the
Antibiotics are the natural substances produced by the microbes that destroy or slow down the growth of the bacteria, whereas antimicrobial agents are the synthetic substances which developed in the laboratory that mimic effects of antibiotics. Selective toxicity means the microbial agents that kill the microbes without killing the host cells. Antimicrobial is divided into two spectrum of antibiotics –
1 Narrow spectrum: - This antibiotic is active against a selected group of the bacteria and will cause less resistance (SRS. n.d)
2 Broad spectrums: - This antibiotic is effective against a wide range of infectious micro-organisms which includes both gram positive and negative bacteria. (SRS. n.d)
The relative …show more content…
-Antibiotic disk of gentamycin, novobiacin and penicillin
-Nutrient agar in petri plate.
-Metric ruler, markers and plastic tweezers.
-Tea candle light, sterile swab, rubbing alcohol and bleach
-Other includes personal protective equipment like an apron, gloves, and safety goggles.
Kirby Bauer diffusion is widely used test for antibiotic effectiveness in certain kind of organisms. The steps I followed to complete this test are-
- First collect all the materials then wash the hands thoroughly and put the personal protective equipment.
- Clean the working surface to prevent from cross contamination.
- Take out the petri plate with nutrient agar in it. Divide the petri plate into three equal sections with permanent marker and label the antibiotic name (gentamicin, novobiacin and penicillin) in each section.
-Remove the lid of the active S epidermis broth, flame the lip of the tube with help of the tea light candle and careful remove the sterile swab and submerge the swab into the active S epidermis broth. -Swab the entire agar plate including the edges and let the active broth absorbed in the