Introduction: The null hypothesis was that there is no difference in dissolved oxygen and primary production with depth, the dissolved oxygen amounts will be constant with differences in depth. The alternative hypothesis is that there is a difference in dissolved oxygen and primary production with depth, the dissolved oxygen amounts will decrease as depth increases.
Methods:
Water samples were incubated in light/dark bottles with no air bubbles in the bottles. DO concentrations were calculated prior to setting the bottles up. The bottles were incubated from Monday at 8:30 a.m. to Tuesday at 1:00 p.m. The bottles were capped underwater so that there is no air in the cap which would affect the dissolved oxygen levels. All the caps on the bottles are taken off and 8 drops of …show more content…
A titrator (syringe) was fully filled with Sodium Thiosulfate and dispensed over the Erlenmeyer flask drop by drop while the flask was being swirled during the titration (after each drop was added) to mix the contents well until the solution was a very faint yellow while it is sat on a white paper towel to see the exact color. The titrator was then set aside to be used again with the same bottle sample that it began with when it was completely full. Eight drops of Starch Indicator Solution were added and swirled in the flask, this caused the sample to turn blue. More titrator is added to the solution in the flask, one drop at a time and swirled until the blue disappeared and the solution turned clear while still being set on a white paper towel to see the exact color. The value on the syringe is then recorded for that sample in parts per million (ppm). The titration solution is refilled without discarding any unused solution in the syringe for the next bottle. The graduated cylinder and funnel is washed out with tap water for the next
Methods:
Water samples were incubated in light/dark bottles with no air bubbles in the bottles. DO concentrations were calculated prior to setting the bottles up. The bottles were incubated from Monday at 8:30 a.m. to Tuesday at 1:00 p.m. The bottles were capped underwater so that there is no air in the cap which would affect the dissolved oxygen levels. All the caps on the bottles are taken off and 8 drops of …show more content…
A titrator (syringe) was fully filled with Sodium Thiosulfate and dispensed over the Erlenmeyer flask drop by drop while the flask was being swirled during the titration (after each drop was added) to mix the contents well until the solution was a very faint yellow while it is sat on a white paper towel to see the exact color. The titrator was then set aside to be used again with the same bottle sample that it began with when it was completely full. Eight drops of Starch Indicator Solution were added and swirled in the flask, this caused the sample to turn blue. More titrator is added to the solution in the flask, one drop at a time and swirled until the blue disappeared and the solution turned clear while still being set on a white paper towel to see the exact color. The value on the syringe is then recorded for that sample in parts per million (ppm). The titration solution is refilled without discarding any unused solution in the syringe for the next bottle. The graduated cylinder and funnel is washed out with tap water for the next