Lab Report Digestion Essay example
Table 2: Reaction of pepsin in 4 different conditions of tubes Tube | Initial pH | Final pH | Estimated digestion | Explanation | 1.Pepsin,HCl | 3 | 3 | +++ | pH 3 caused from the HCl presence and is optimum for pepsin activity so the amount of protein digestion is highest among others. | 2.Pepsin,water | 5 | 6 | ++ | The pH is 5 which higher than optimum pH of pepsin and pepsin is not in acidic environment. Thus, the amount of protein digestion is lower than tube 1. | 3.HCl,water | 3 | 3 | + | The pH is already optimum for pepsin but there is absence of pepsin. Thus, the amount of protein digestion is the lowest. | 4.Pepsin,NaOH | 14 | 14 | - | Pepsin work best in acidic environment, not alkaline. Thus, there is no protein digestion occurred. |
5.0. POST-LAB QUESTION
5.1. Salivary Digestion of Carbohydrates 1. Function of mucin in the mouth:
Acts as lubricant for easier swallowing and protects the mouth lining from abrasion. 2. Indicate the relative amounts of starch and maltose after incubation.
3. What in vivo (in the body) situation is simulated by the conditions in tube 4?
The hydrolysis of carbohydrates by the act of ptyalin is inhibited. 4. Does ptyalin hydrolysis of carbohydrate continue in the