Lab Report A Number Of Tests And Results Will Be Discussed Essays

802 Words Apr 30th, 2016 4 Pages
In this lab report a number of tests and results will be discussed. Unknown labs are performed for many reasons ranging from figuring out the causative agent in a diseased patient, to knowing the correct microorganism to use when making certain foods and antibiotics. Unknown labs are a key to testing the knowledge a student has gained throughout the course.
Materials:
Microscope Bunsen Burner Beaker Test Tube Holder Test Tube Clothes pin Striker Loops and Needles Distilled Water Crystal Violet Alcohol Safranin Iodine Methylene Blue Hydrogen Peroxide Reagent A (sulfanilic acid)
Reagent B (dimethyl-alpha-napthylamine) Kovacs Reagent EMb plate Blood plate MSA plate Starch plate Litmus milk tube Nutrient agar Slant Agar Plates Nutrient Broth MR-VP Broths Citrate Tube
Barritt’s Reagent A (alpha-naphthol) Barritt’s Reagent B (KOH) Cotton Swab Pipettes Methyl Red bibulous paper Glucose tube Lactose tube
Dextrouse tube Microscope slide Cover slip

On the first day of observations, an unknown test tube and plate labeled with the letter “H” was handed out by the lab instructor. The first procedure that needed to be done to identify the organism was a simple stain. Simple staining determines the micrograms morphology. The next procedure that had to be performed was a gram stain. Gram staining determines whether the microorganism is gram positive or gram negative. After doing these procedures it was determined that the organism was a gram…

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